Mary Shamya Arokiarajan, J. John Christopher, S.A. Wasim Akram, N. Zaheer Ahmed, Rampratap Meena, K. Kabiruddin Ahmed
{"title":"Phyto-chemical, analytical evaluation and biological attributes of rare medicinal species of flowering shrub Bauhinia racemosa L.","authors":"Mary Shamya Arokiarajan, J. John Christopher, S.A. Wasim Akram, N. Zaheer Ahmed, Rampratap Meena, K. Kabiruddin Ahmed","doi":"10.1007/s13596-024-00761-2","DOIUrl":null,"url":null,"abstract":"<div><p>The current investigation compared the efficacy of an ethanol extract of <i>B. racemosa</i> L. leaf against standard bacterial and fungal cultures. Quantitative phytochemical analysis of Phenol, Flavonoid and Tanin was studied by the appropriate methods; Heavy metal test was done by Atomic Absorption Spectrometer; Antimicrobial activity was done by disc diffusion and agar well diffusion method; MIC through 96 well method and antioxidant assay was performed by DPPH and ABTS. Ethanol extract of <i>B. racemosa</i> L. showed significantly higher inhibitory effect against <i>E. coli</i>, <i>S. aureus</i> and <i>B. cereus</i>, and moderate antimicrobial activity against <i>K. pneumoniae</i>, but it was inactive on fungal strain at lower concentrations. The minimum bactericidal concentration of <i>B. racemosa</i> L. extract against the pathogenic bacteria tested was 1.25 mg/ml for <i>P. aeruginosa</i>, 2.5 mg/ml for <i>E. coli</i> and 5 mg/ml for <i>B. cereus</i>, <i>K. pnuemoniae</i>, and <i>S. aureus</i>. The extract has exhibited antioxidant activity which was evaluated by DPPH and ABTS as 61.61 ± 0.61 and 64.45 ± 0.49% of inhibition in 250 µg/ml concentration. Prominent phytochemical bioconstituents, as determined by phytochemical investigation, comprise flavonoids (8.712 ± 0.7 mg/g Rutin equivalents), tannins (2.930 ± 0.73 mg/g Tannic acid equivalents) and phenol (12.06 ± 0.25 mg/g Gallic acid equivalents) etc. Lethality experiment was performed by using brine shrimp to determine the cytotoxicity of plant extract and the substantial mortality rate observed as LC50 = 22.8435 µg/mL. The result of fluorescence analysis showed various shades of green and brown fluorescence in visible light and various shades of green, blue and brown were found under UV light. HPTLC, FTIR and GCMS were done to find out the bioactive phytocompounds.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"1181 - 1203"},"PeriodicalIF":1.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-024-00761-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
The current investigation compared the efficacy of an ethanol extract of B. racemosa L. leaf against standard bacterial and fungal cultures. Quantitative phytochemical analysis of Phenol, Flavonoid and Tanin was studied by the appropriate methods; Heavy metal test was done by Atomic Absorption Spectrometer; Antimicrobial activity was done by disc diffusion and agar well diffusion method; MIC through 96 well method and antioxidant assay was performed by DPPH and ABTS. Ethanol extract of B. racemosa L. showed significantly higher inhibitory effect against E. coli, S. aureus and B. cereus, and moderate antimicrobial activity against K. pneumoniae, but it was inactive on fungal strain at lower concentrations. The minimum bactericidal concentration of B. racemosa L. extract against the pathogenic bacteria tested was 1.25 mg/ml for P. aeruginosa, 2.5 mg/ml for E. coli and 5 mg/ml for B. cereus, K. pnuemoniae, and S. aureus. The extract has exhibited antioxidant activity which was evaluated by DPPH and ABTS as 61.61 ± 0.61 and 64.45 ± 0.49% of inhibition in 250 µg/ml concentration. Prominent phytochemical bioconstituents, as determined by phytochemical investigation, comprise flavonoids (8.712 ± 0.7 mg/g Rutin equivalents), tannins (2.930 ± 0.73 mg/g Tannic acid equivalents) and phenol (12.06 ± 0.25 mg/g Gallic acid equivalents) etc. Lethality experiment was performed by using brine shrimp to determine the cytotoxicity of plant extract and the substantial mortality rate observed as LC50 = 22.8435 µg/mL. The result of fluorescence analysis showed various shades of green and brown fluorescence in visible light and various shades of green, blue and brown were found under UV light. HPTLC, FTIR and GCMS were done to find out the bioactive phytocompounds.
期刊介绍:
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.