Phyto-chemical, analytical evaluation and biological attributes of rare medicinal species of flowering shrub Bauhinia racemosa L.

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Advances in Traditional Medicine Pub Date : 2024-07-01 DOI:10.1007/s13596-024-00761-2
Mary Shamya Arokiarajan, J. John Christopher, S.A. Wasim Akram, N. Zaheer Ahmed, Rampratap Meena, K. Kabiruddin Ahmed
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Abstract

The current investigation compared the efficacy of an ethanol extract of B. racemosa L. leaf against standard bacterial and fungal cultures. Quantitative phytochemical analysis of Phenol, Flavonoid and Tanin was studied by the appropriate methods; Heavy metal test was done by Atomic Absorption Spectrometer; Antimicrobial activity was done by disc diffusion and agar well diffusion method; MIC through 96 well method and antioxidant assay was performed by DPPH and ABTS. Ethanol extract of B. racemosa L. showed significantly higher inhibitory effect against E. coli, S. aureus and B. cereus, and moderate antimicrobial activity against K. pneumoniae, but it was inactive on fungal strain at lower concentrations. The minimum bactericidal concentration of B. racemosa L. extract against the pathogenic bacteria tested was 1.25 mg/ml for P. aeruginosa, 2.5 mg/ml for E. coli and 5 mg/ml for B. cereus, K. pnuemoniae, and S. aureus. The extract has exhibited antioxidant activity which was evaluated by DPPH and ABTS as 61.61 ± 0.61 and 64.45 ± 0.49% of inhibition in 250 µg/ml concentration. Prominent phytochemical bioconstituents, as determined by phytochemical investigation, comprise flavonoids (8.712 ± 0.7 mg/g Rutin equivalents), tannins (2.930 ± 0.73 mg/g Tannic acid equivalents) and phenol (12.06 ± 0.25 mg/g Gallic acid equivalents) etc. Lethality experiment was performed by using brine shrimp to determine the cytotoxicity of plant extract and the substantial mortality rate observed as LC50 = 22.8435 µg/mL. The result of fluorescence analysis showed various shades of green and brown fluorescence in visible light and various shades of green, blue and brown were found under UV light. HPTLC, FTIR and GCMS were done to find out the bioactive phytocompounds.

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稀有药用花灌木洋紫荆(Bauhinia racemosa L.)的植物化学、分析评价和生物属性
目前的研究比较了一种乙醇提取物对标准细菌和真菌培养物的功效。采用适当的方法对酚、类黄酮和丹宁进行了植物化学定量分析;采用原子吸收光谱仪进行了重金属测试;采用盘式扩散法和琼脂井扩散法进行了抗菌活性测试;采用 96 孔法进行了 MIC 测试;采用 DPPH 和 ABTS 进行了抗氧化测试。结果表明,乙醇提取物对大肠杆菌、金黄色葡萄球菌和蜡样芽孢杆菌有较高的抑制作用,对肺炎双球菌有中等程度的抗菌活性,但在较低浓度下对真菌菌株无活性。对铜绿假单胞菌、大肠杆菌、蜡样芽胞杆菌、肺炎双球菌和金黄色葡萄球菌的最低杀菌浓度分别为 1.25 毫克/毫升、2.5 毫克/毫升和 5 毫克/毫升。经 DPPH 和 ABTS 评估,在 250 µg/ml 浓度下,提取物的抗氧化活性分别为 61.61 ± 0.61% 和 64.45 ± 0.49%。通过植物化学调查确定的主要植物化学生物成分包括类黄酮(8.712 ± 0.7 毫克/克芦丁当量)、单宁(2.930 ± 0.73 毫克/克单宁酸当量)和酚(12.06 ± 0.25 毫克/克没食子酸当量)等。使用盐水虾进行了致死实验,以确定植物提取物的细胞毒性,观察到 LC50 = 22.8435 µg/mL 的大幅死亡率。荧光分析结果表明,在可见光下,植物提取物发出深浅不同的绿色和棕色荧光;在紫外光下,植物提取物发出深浅不同的绿色、蓝色和棕色荧光。通过 HPTLC、傅立叶变换红外光谱和气相色谱联用技术,找出了具有生物活性的植物化合物。
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
期刊最新文献
In-vitro and in-silico evaluation of anticancer potential of Oxalis corniculata Effect of a traditional herbal formulation (Azaraghi Majoon) on opium use disorder in male rats Phyto-chemical, analytical evaluation and biological attributes of rare medicinal species of flowering shrub Bauhinia racemosa L. Validation of the constitution in Chinese medicine questionnaires with the newly developed blood-vacuity category: a rare Taiwanese case study Correction to: Unveiling the potential antibacterial mechanism of Melaleuca cajuputi leaf extract by cell morphology studies and molecular docking analysis
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