Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis

Prostaglandins, leukotrienes, and medicine Pub Date : 1987-05-01 DOI:10.1016/0262-1746(87)90067-9

F.J. Zijlstra , A.M.W.C. van den Broek , J.E. Vincent , P.P.H.M. Diderich , A.M.C. op de Hoek-Fez , M. Claeys

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引用次数: 6

Abstract

Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with ¹⁴C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B₄ (LTB₄) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F_1α (6-keto-PGF_1α and thromboxane B₂ (TxB₂). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B₄ (20-OH-LTB₄). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB₄, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.

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持续动态腹膜透析患者腹膜炎期间巨噬细胞形成白三烯B4、20-羟基白三烯B4及其他花生四烯酸代谢物的研究

用14c -花生四烯酸标记3例连续动态腹膜透析(CAPD)患者腹膜炎期间不同时间透析液中分离的巨噬细胞，并用钙离子载体A23187刺激巨噬细胞。5-脂氧合酶活性形成的主要代谢产物是白三烯B4 (LTB4)和5-羟基-6,9,11,14-二十碳四烯酸(5-HETE)。环加氧酶代谢物较少，洗脱时间介于6-酮-前列腺素F1α(6-酮- pgf1 α)和血栓素B2 (TxB2)之间的主要化合物。该物质经GC-MS分离鉴定为20-羟基白三烯B4 (20-OH-LTB4)。这表明从CAPD获得的人腹腔巨噬细胞不仅产生白三烯和前列腺素，而且还产生LTB4的ω-羟化酶产物，该产物已被证明存在于多形核白细胞中。该酶的活性与腹膜炎的严重程度无关。

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Prostaglandins, leukotrienes, and medicine

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