Serum microRNA-125b-5p expression in patients with dilated cardiomyopathy combined with heart failure and its effect on myocardial fibrosis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-12-01 Epub Date: 2024-07-18 DOI:10.1080/14017431.2024.2373083

Yingjie Zhang, Daqing Deng, Quan Huang, Jiaru Wu, Yi Xiang, Boqing Ou

{"title":"Serum microRNA-125b-5p expression in patients with dilated cardiomyopathy combined with heart failure and its effect on myocardial fibrosis.","authors":"Yingjie Zhang, Daqing Deng, Quan Huang, Jiaru Wu, Yi Xiang, Boqing Ou","doi":"10.1080/14017431.2024.2373083","DOIUrl":null,"url":null,"abstract":"Objective: This paper was performed to decipher the serum microRNA (miR)-125b-5p expression in patients with dilated cardiomyopathy (DCM) combined with heart failure (HF) and its effect on myocardial fibrosis.Methods: Serum miR-125b-5p expression, LVEDD, LVESD, LVEF, LVFS, and NT-proBNP levels were evaluated in clinical samples. A rat DCM model was established by continuous intraperitoneal injection of adriamycin and treated with miR-125b-5p agomir and its negative control. Cardiac function, serum TNF-α, hs-CRP, and NT-proBNP levels, pathological changes in myocardial tissues, cardiomyocyte apoptosis, and the expression levels of miR-125b-5p and fibrosis-related factors were detected in rats.Results: In comparison to the control group, the case group had higher levels of LVEDD, LVESD, and NT-pro-BNP, and lower levels of LVEF, LVFS, and miR-125b-5p expression levels. Overexpression of miR-125b-5p effectively led to the improvement of cardiomyocyte hypertrophy and collagen arrangement disorder in DCM rats, the reduction of blue-stained collagen fibers in the interstitial myocardium, the reduction of the levels of TNF-α, hs-CRP, and NT-proBNP and the expression levels of TGF-1β, Collagen I, and α-SMA, and the reduction of the number of apoptosis in cardiomyocytes.Conclusion: Overexpression of miR-125b-5p is effective in ameliorating myocardial fibrosis.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/14017431.2024.2373083","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This paper was performed to decipher the serum microRNA (miR)-125b-5p expression in patients with dilated cardiomyopathy (DCM) combined with heart failure (HF) and its effect on myocardial fibrosis.

Methods: Serum miR-125b-5p expression, LVEDD, LVESD, LVEF, LVFS, and NT-proBNP levels were evaluated in clinical samples. A rat DCM model was established by continuous intraperitoneal injection of adriamycin and treated with miR-125b-5p agomir and its negative control. Cardiac function, serum TNF-α, hs-CRP, and NT-proBNP levels, pathological changes in myocardial tissues, cardiomyocyte apoptosis, and the expression levels of miR-125b-5p and fibrosis-related factors were detected in rats.

Results: In comparison to the control group, the case group had higher levels of LVEDD, LVESD, and NT-pro-BNP, and lower levels of LVEF, LVFS, and miR-125b-5p expression levels. Overexpression of miR-125b-5p effectively led to the improvement of cardiomyocyte hypertrophy and collagen arrangement disorder in DCM rats, the reduction of blue-stained collagen fibers in the interstitial myocardium, the reduction of the levels of TNF-α, hs-CRP, and NT-proBNP and the expression levels of TGF-1β, Collagen I, and α-SMA, and the reduction of the number of apoptosis in cardiomyocytes.

Conclusion: Overexpression of miR-125b-5p is effective in ameliorating myocardial fibrosis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

扩张型心肌病合并心力衰竭患者血清 microRNA-125b-5p 的表达及其对心肌纤维化的影响

研究目的本文旨在解读扩张型心肌病（DCM）合并心力衰竭（HF）患者血清microRNA（miR）-125b-5p的表达及其对心肌纤维化的影响：方法：评估临床样本中血清 miR-125b-5p 表达、LVEDD、LVESD、LVEF、LVFS 和 NT-proBNP 水平。通过连续腹腔注射阿霉素建立了大鼠 DCM 模型，并用 miR-125b-5p 激动剂及其阴性对照进行治疗。检测大鼠心功能、血清 TNF-α、hs-CRP 和 NT-proBNP 水平、心肌组织病理变化、心肌细胞凋亡以及 miR-125b-5p 和纤维化相关因子的表达水平：结果：与对照组相比，病例组的 LVEDD、LVESD 和 NT-pro-BNP 水平较高，而 LVEF、LVFS 和 miR-125b-5p 表达水平较低。过表达miR-125b-5p能有效改善DCM大鼠心肌细胞肥大和胶原排列紊乱，减少心肌间质蓝染胶原纤维，降低TNF-α、hs-CRP和NT-proBNP水平以及TGF-1β、胶原I和α-SMA的表达水平，减少心肌细胞凋亡数量：结论：过表达 miR-125b-5p 能有效改善心肌纤维化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.