Phylogenetically diverse bacterial species produce histamine

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2024-07-17 DOI:10.1016/j.syapm.2024.126539
Kristen A. Engevik , Amber Hazzard , Brenton Puckett , Kathleen M. Hoch , Sigmund J. Haidacher , Anthony M. Haag , Jennifer K. Spinler , James Versalovic , Melinda A. Engevik , Thomas D. Horvath
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Abstract

Histamine is an important biogenic amine known to impact a variety of patho-physiological processes ranging from allergic reactions, gut-mediated anti-inflammatory responses, and neurotransmitter activity. Histamine is found both endogenously within specialized host cells and exogenously in microbes. Exogenous histamine is produced through the decarboxylation of the amino acid L-histidine by bacterial-derived histidine decarboxylase enzymes. To investigate how widespread histamine production is across bacterial species, we examined 102,018 annotated genomes in the Integrated Microbial Genomes Database and identified 3,679 bacterial genomes (3.6 %) which possess the enzymatic machinery to generate histamine. These bacteria belonged to 10 phyla: Bacillota, Bacteroidota, Actinomycetota, Pseudomonadota, Lentisphaerota, Fusobacteriota, Armatimonadota, Cyanobacteriota, Thermodesulfobacteriota, and Verrucomicrobiota. The majority of the identified bacteria were terrestrial or aquatic in origin, although several bacteria originated in the human gut microbiota. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted metabolomics to confirm our genome discoveries correlated with L-histidine-to-histamine conversion in a chemically defined bacterial growth medium by a cohort of select environmental and human gut bacteria. We found that environmental microbes Vibrio harveyi, Pseudomonas fluorescens and Streptomyces griseus generated considerable levels of histamine (788 – 8,730 ng/mL). Interestingly, we found higher concentrations of histamine produced by gut-associated Fusobacterium varium, Clostridium perfringens, Limosilactobacillus reuteri and Morganella morganii (8,510––82,400 ng/mL). This work expands our knowledge of histamine production by diverse microbes.

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不同系统发育的细菌物种都会产生组胺。
组胺是一种重要的生物胺,已知会影响过敏反应、肠道介导的抗炎反应和神经递质活动等多种病理生理过程。组胺既存在于特化的宿主细胞内源性,也存在于微生物的外源性。外源性组胺是通过源于细菌的组氨酸脱羧酶将氨基酸 L-组氨酸脱羧而产生的。为了研究组胺的产生在细菌物种中的广泛程度,我们研究了综合微生物基因组数据库(Integrated Microbial Genomes Database)中的102,018个注释基因组,发现3,679个细菌基因组(3.6%)拥有产生组胺的酶机制。这些细菌属于 10 个门类:这些细菌属于 10 个门类:芽孢杆菌门、类杆菌门、放线菌门、假单胞菌门、扁桃体杆菌门、镰刀菌门、臂杆菌门、蓝藻菌门、热硫化杆菌门和疣菌门。虽然有几种细菌来源于人类肠道微生物群,但大多数被鉴定的细菌来源于陆生或水生生物。我们使用基于液相色谱-串联质谱(LC-MS/MS)的靶向代谢组学来证实我们的基因组发现与精选的环境细菌和人类肠道细菌在化学定义的细菌生长培养基中将左旋组氨酸转化为组胺有关。我们发现,环境微生物哈维弧菌、荧光假单胞菌和灰葡萄孢链霉菌产生了相当高水平的组胺(788 - 8730 纳克/毫升)。有趣的是,我们发现肠道相关的变异镰刀菌、产气荚膜梭菌、Limosilactobacillus reuteri和摩根菌产生的组胺浓度更高(8510-82400纳克/毫升)。这项研究拓展了我们对多种微生物产生组胺的认识。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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