Murine glial protrusion transcripts predict localized Drosophila glial mRNAs involved in plasticity.

IF 7.4 1区 生物学 Q1 CELL BIOLOGY Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-07-22 DOI:10.1083/jcb.202306152
Jeffrey Y Lee, Dalia S Gala, Maria Kiourlappou, Julia Olivares-Abril, Jana Joha, Joshua S Titlow, Rita O Teodoro, Ilan Davis
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Abstract

The polarization of cells often involves the transport of specific mRNAs and their localized translation in distal projections. Neurons and glia are both known to contain long cytoplasmic processes, while localized transcripts have only been studied extensively in neurons, not glia, especially in intact nervous systems. Here, we predict 1,740 localized Drosophila glial transcripts by extrapolating from our meta-analysis of seven existing studies characterizing the localized transcriptomes and translatomes of synaptically associated mammalian glia. We demonstrate that the localization of mRNAs in mammalian glial projections strongly predicts the localization of their high-confidence Drosophila homologs in larval motor neuron-associated glial projections and are highly statistically enriched for genes associated with neurological diseases. We further show that some of these localized glial transcripts are specifically required in glia for structural plasticity at the nearby neuromuscular junction synapses. We conclude that peripheral glial mRNA localization is a common and conserved phenomenon and propose that it is likely to be functionally important in disease.

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小鼠神经胶质突起转录本预测了果蝇神经胶质 mRNA 的定位,这些 mRNA 与可塑性有关。
细胞的极化往往涉及特定 mRNA 的运输及其在远端突起中的定位翻译。众所周知,神经元和胶质细胞都含有长的细胞质过程,而定位转录本只在神经元中得到了广泛研究,而在胶质细胞中却没有,尤其是在完整的神经系统中。在这里,我们通过对现有的七项研究进行荟萃分析,预测了 1740 个果蝇神经胶质的定位转录本,这些研究描述了与突触相关的哺乳动物神经胶质的定位转录本组和转译组的特征。我们证明,哺乳动物神经胶质突起中 mRNA 的定位强烈预测了其高置信度果蝇同源物在幼虫运动神经元相关神经胶质突起中的定位,并且在统计学上高度富集了与神经系统疾病相关的基因。我们进一步发现,这些定位的神经胶质转录本中有一些是神经胶质对附近神经肌肉接头突触结构可塑性的特异性要求。我们的结论是,外周神经胶质 mRNA 定位是一种常见的保守现象,并认为它在疾病中可能具有重要功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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