A novel long noncoding RNA ENST00000597482 serves as a potential biomarker for disease activity and diagnosis of systemic lupus erythematosus.

IF 1.9 4区 医学 Q3 RHEUMATOLOGY Lupus Pub Date : 2024-09-01 Epub Date: 2024-07-22 DOI:10.1177/09612033241266988
Cuicui Wang, Shiwen Yuan, Yanting Zeng, Weinian Li, Jinghua Ye, Fangfei Li, Zhixiang He, Yi Chen, Xiaojun Lin, Liuqin Liang, Hanshi Xu, Xiaoyan Cai
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Abstract

Objectives: Emerging evidence indicate that long noncoding RNAs (lncRNAs) may play an important role in the pathogenesis of systemic lupus erythematosus (SLE) however, the contribution of lncRNAs to SLE remains largely unclear. Our study aimed to explore the lncRNA expression profiles in peripheral blood mononuclear cells (PBMCs) from SLE patients.

Methods: LncRNA sequencing was used to detect differentially expressed genes in PBMCs from 5 SLE-MIX samples and 3 healthy controls (HC)-MIX samples, and the expression of selected lncRNAs was further verified by real-time quantitative polymerase chain reaction (RT‒qPCR). The correlation of lncRNA expression with laboratory indicators as well the SLE disease activity index 2000 (SLEDAI‒2K) score from 72 SLE patients was assessed by Spearman's test. The association between lncRNA ENST00000597482 and organ involvement in SLE patients was determined by the Mann‒Whitney U test. Moreover, lymphocyte subsets in peripheral blood from SLE patients were measured by flow cytometry. In addition, the diagnostic value of lncRNAs in predicting SLE was evaluated by receiver operating characteristic (ROC) curve analysis.

Results: The lncRNA expression profiles demonstrated 218 differentially expressed lncRNAs, including 121 upregulated genes and 97 downregulated genes, in PBMCs from SLE patients compared to HCs. Among the 10 candidate genes selected, only lncRNA ENST00000597482, which was lower in SLE PBMCs than in HCs, was consistent with the sequencing results. LncRNA ENST00000597482 expression was negatively correlated with SLEDAI-2K score and the titres of ANA antibodies and anti-double-stranded DNA (anti-dsDNA) antibodies. Of note, SLE patients with lower expression of lncRNA ENST00000597482 were prone to develop organ involvement. Furthermore, lncRNA ENST00000597482 exhibited potential diagnostic value in differentiating SLE patients from HCs.

Conclusions: LncRNA ENST00000597482 expression was lower in PBMCs from SLE patients than HCs and was negatively correlated with the SLEDAI-2K score and autoantibody titres. In addition, lncRNA ENST00000597482 could act as a novel biomarker for disease activity and diagnosis of SLE.

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一种新型长非编码 RNA ENST00000597482 可作为疾病活动和诊断系统性红斑狼疮的潜在生物标记物。
目的:新的证据表明,长非编码RNA(lncRNA)可能在系统性红斑狼疮(SLE)的发病机制中扮演重要角色,但是,lncRNA对SLE的贡献在很大程度上仍不清楚。我们的研究旨在探索系统性红斑狼疮患者外周血单核细胞(PBMC)中的lncRNA表达谱:方法:采用 LncRNA 测序技术检测 5 个 SLE-MIX 样本和 3 个健康对照(HC)-MIX 样本的 PBMC 中差异表达的基因,并通过实时定量聚合酶链反应(RT-qPCR)进一步验证所选 lncRNA 的表达。通过斯皮尔曼检验评估了lncRNA表达与实验室指标以及72名系统性红斑狼疮患者的系统性红斑狼疮疾病活动指数2000(SLEDAI-2K)评分的相关性。lncRNA ENST00000597482 与系统性红斑狼疮患者器官受累之间的关系通过 Mann-Whitney U 检验来确定。此外,还通过流式细胞术测量了系统性红斑狼疮患者外周血中的淋巴细胞亚群。此外,还通过接收者操作特征曲线(ROC)分析评估了lncRNA在预测系统性红斑狼疮方面的诊断价值:结果:lncRNA表达谱显示,与HCs相比,系统性红斑狼疮患者的PBMC中有218个不同表达的lncRNA,包括121个上调基因和97个下调基因。在选出的10个候选基因中,只有lncRNA ENST00000597482在系统性红斑狼疮患者PBMCs中的表达量低于HCs,这与测序结果一致。LncRNA ENST00000597482的表达与SLEDAI-2K评分、ANA抗体滴度和抗双链DNA(anti-dsDNA)抗体滴度呈负相关。值得注意的是,lncRNA ENST00000597482表达量较低的系统性红斑狼疮患者容易出现器官受累。此外,lncRNA ENST00000597482在区分系统性红斑狼疮患者与HCs方面具有潜在的诊断价值:LncRNA ENST00000597482在系统性红斑狼疮患者PBMCs中的表达低于HCs,且与SLEDAI-2K评分和自身抗体滴度呈负相关。此外,lncRNA ENST00000597482 可作为疾病活动和系统性红斑狼疮诊断的新型生物标记物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
期刊最新文献
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