A novel MAP kinase-interacting protein MoSmi1 regulates development and pathogenicity in Magnaporthe oryzae.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-07-01 DOI:10.1111/mpp.13493
Yu Wang, Xinyue Cui, Junlian Xiao, Xiaoru Kang, Jinmei Hu, Zhicheng Huang, Na Li, Chuyu Yang, Yuemin Pan, Shulin Zhang
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Abstract

The cell wall is the first barrier against external adversity and plays roles in maintaining normal physiological functions of fungi. Previously, we reported a nucleosome assembly protein, MoNap1, in Magnaporthe oryzae that plays a role in cell wall integrity (CWI), stress response, and pathogenicity. Moreover, MoNap1 negatively regulates the expression of MoSMI1 encoded by MGG_03970. Here, we demonstrated that deletion of MoSMI1 resulted in a significant defect in appressorium function, CWI, cell morphology, and pathogenicity. Further investigation revealed that MoSmi1 interacted with MoOsm1 and MoMps1 and affected the phosphorylation levels of MoOsm1, MoMps1, and MoPmk1, suggesting that MoSmi1 regulates biological functions by mediating mitogen-activated protein kinase (MAPK) signalling pathway in M. oryzae. In addition, transcriptome data revealed that MoSmi1 regulates many infection-related processes in M. oryzae, such as membrane-related pathway and oxidation reduction process. In conclusion, our study demonstrated that MoSmi1 regulates CWI by mediating the MAPK pathway to affect development and pathogenicity of M. oryzae.

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一种新的与 MAP 激酶相互作用的蛋白 MoSmi1 可调控木格氏真菌的发育和致病性。
细胞壁是抵御外部逆境的第一道屏障,在维持真菌正常生理功能方面发挥作用。此前,我们报道了一种核糖体组装蛋白--MoNap1,它在木格氏真菌的细胞壁完整性(CWI)、应激反应和致病性中发挥作用。此外,MoNap1 负向调节由 MGG_03970 编码的 MoSMI1 的表达。在这里,我们证明了缺失 MoSMI1 会导致贴壁功能、CWI、细胞形态和致病性的显著缺陷。进一步研究发现,MoSmi1与MoOsm1和MoMps1相互作用,并影响MoOsm1、MoMps1和MoPmk1的磷酸化水平,表明MoSmi1通过介导有丝分裂原激活蛋白激酶(MAPK)信号通路调控M.此外,转录组数据显示,MoSmi1 还调控着许多与 M. oryzae 感染相关的过程,如膜相关途径和氧化还原过程。总之,我们的研究表明,MoSmi1 通过介导 MAPK 通路来调控 CWI,从而影响 M. oryzae 的发育和致病性。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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