Extracellular flux assay (Seahorse assay): Diverse applications in metabolic research across biological disciplines

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecules and Cells Pub Date : 2024-08-01 DOI:10.1016/j.mocell.2024.100095

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Abstract

Metabolic networks are fundamental to cellular processes, driving energy production, biosynthesis, redox regulation, and cellular signaling. Recent advancements in metabolic research tools have provided unprecedented insights into cellular metabolism. Among these tools, the extracellular flux analyzer stands out for its real-time measurement of key metabolic parameters: glycolysis, mitochondrial respiration, and fatty acid oxidation, leading to its widespread use. This review provides a comprehensive summary of the basic principles and workflow of the extracellular flux assay (the Seahorse assay) and its diverse applications. We highlight the assay's versatility across various biological models, including cancer cells, immunocytes, Caenorhabditis elegans, tissues, isolated mitochondria, and three-dimensional structures such as organoids, and summarize key considerations for using extracellular flux assay in these models. Additionally, we discuss the limitations of the Seahorse assay and propose future directions for its development. This review aims to enhance the understanding of extracellular flux assay and its significance in biological studies.

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细胞外通量测定法（海马测定法）：生物学科代谢研究中的多样化应用。

代谢网络是细胞过程的基础，驱动着能量生产、生物合成、氧化还原调节和细胞信号传导。新陈代谢研究工具的最新进展为细胞新陈代谢提供了前所未有的洞察力。在这些工具中，细胞外通量分析仪因其可实时测量糖酵解、线粒体呼吸和脂肪酸氧化（FAO）等关键代谢参数而脱颖而出，并得到广泛应用。本综述全面总结了细胞外通量测定（海马测定）的基本原理和工作流程及其各种应用。我们强调了该测定法在各种生物模型中的通用性，包括癌细胞、免疫细胞、优雅小鼠、组织、离体线粒体和三维结构（如有机体），并总结了在这些模型中使用细胞外通量测定法的主要注意事项。此外，我们还讨论了海马测定的局限性，并提出了其未来的发展方向。本综述旨在加深对细胞外通量测定及其在生物学研究中的意义的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.

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