Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in vitro embryos?

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-07-01 DOI:10.1111/rda.14672

Rafaella Silva Andrade, Dircéia Aparecida da Costa Custódio, Leonardo Silva Fernandes do Vale, Érika Aparecida Oliveira, Andrey Pereira Lage, Elaine Maria Seles Dorneles

{"title":"Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in vitro embryos?","authors":"Rafaella Silva Andrade, Dircéia Aparecida da Costa Custódio, Leonardo Silva Fernandes do Vale, Érika Aparecida Oliveira, Andrey Pereira Lage, Elaine Maria Seles Dorneles","doi":"10.1111/rda.14672","DOIUrl":null,"url":null,"abstract":"<p><p>Animal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect Brucella spp. in the ovarian follicular fluid of brucellosis-positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from Brucella-positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus Brucella (bcsp31 gene). All 47 bovine aspirates were negative for Brucella spp. 0.00% (95% CI: 0.00-4.00%). Our results demonstrated that Brucella spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that Brucella spp.-infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis-seropositive cows to transfer embryos to heifers/cows not infected by Brucella, aiming to produce calves free of the infection.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction in Domestic Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/rda.14672","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Animal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect Brucella spp. in the ovarian follicular fluid of brucellosis-positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from Brucella-positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus Brucella (bcsp31 gene). All 47 bovine aspirates were negative for Brucella spp. 0.00% (95% CI: 0.00-4.00%). Our results demonstrated that Brucella spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that Brucella spp.-infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis-seropositive cows to transfer embryos to heifers/cows not infected by Brucella, aiming to produce calves free of the infection.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

使用布鲁氏菌病血清反应阳性奶牛的卵巢滤泡液生产体外胚胎是否安全？

动物繁殖生物技术是畜牧业技术进步的重要工具，因为通过这些技术可以选择具有繁殖潜力的优质雌性和雄性动物；然而，偏好生殖系统的传染病可能会阻碍这些技术的使用。因此，本研究旨在检测布鲁氏菌阳性牛卵泡液中的布鲁氏菌属。研究人员对来自布鲁氏菌阳性牛群、布鲁氏菌检测呈阳性的 47 头牛的卵巢滤泡液进行了 PCR 检测。PCR 中使用的引物是布鲁氏菌属（bcsp31 基因）的特异性引物。所有 47 头牛的抽吸物中布鲁氏菌均为阴性，占 0.00%（95% CI：0.00-4.00%）。我们的研究结果表明，血清反应阳性奶牛的卵泡液中不含布鲁氏杆菌，这表明受布鲁氏杆菌感染的奶牛可用于利用卵泡抽吸物进行生殖生物技术研究。未来的研究需要更精确地评估利用这些来自布鲁氏菌血清反应阳性奶牛的卵母细胞将胚胎移植到未感染布鲁氏菌的小母牛/奶牛体内的可行性和安全性，目的是培育出未感染布鲁氏菌的小牛。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.