Association of FAS -670 G > A and FASLG -844 T > C polymorphisms and their circulating serum soluble markers with risk of type 2 diabetes: a case control study
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引用次数: 0
Abstract
Background
Type 2 Diabetes (T2D) is catalyzed by enhanced oxidative stress which induces pancreatic beta cell apoptosis. Genetic variations in extrinsic death pathway genes like FAS and FASLG might alter the apoptotic activity and cause individuals susceptible to T2D.
Objective
The present study aimed to evaluate the oxidative stress markers, the association of FAS -670 G > A and FASLG -844 T > C polymorphisms and their serum soluble levels in type 2 diabetes.
Methods
Serum nitrates/nitrites and oxidative DNA damage levels were estimated by Griess reagent assay and Comet assay, respectively. Genotyping of FAS -670 G > A and FASLG -844 T > C polymorphisms were determined by PCR–RFLP method. Serum soluble FAS and soluble FASLG levels were estimated by ELISA. Statistical analyses were performed using Open-epi, SNPStats and SPSS software. Gene–gene and gene-epidemiological interactions were performed using MDR software.
Results
Nitrates/nitrites and oxidative DNA damage levels were significantly higher in T2D patients compared to controls. Molecular analysis has revealed the association of FAS -670 GG and FASLG -844 CC genotypes with increased risk of T2D. In addition, it was found that T2D patients had significantly lower serum soluble FAS levels and higher serum soluble FASLG levels. Further, MDR gene–gene interaction analysis has demonstrated strong interaction between the genes, whereas gene-epidemiological interactions have shown additive effects on the disease phenotype.
Conclusion
The higher levels of nitrates/nitrites and oxidative DNA damage in individuals with T2D highlight the role of oxidative stress in T2D development. Additionally, the association between the FAS -670 G > A and FASLG -844 T > C polymorphisms and elevated risk of T2D underscores the plausible impact of genetic variants on T2D susceptibility.
期刊介绍：
International Journal of Diabetes in Developing Countries is the official journal of Research Society for the Study of Diabetes in India. This is a peer reviewed journal and targets a readership consisting of clinicians, research workers, paramedical personnel, nutritionists and health care personnel working in the field of diabetes. Original research articles focusing on clinical and patient care issues including newer therapies and technologies as well as basic science issues in this field are considered for publication in the journal. Systematic reviews of interest to the above group of readers are also accepted.
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