Arbuscular mycorrhizal fungi affect early phenological stages of three secondary vegetation species in a temperate forest

Abstract

The relationship between arbuscular mycorrhizal fungi (AMF) and secondary vegetation (SV) species at early phenological stages is critical for the successful establishment of these plants on disturbance sites in temperate forests. The main objective of this research is to evaluate the effect of AMF colonization on the early phenological stages (germination and early growth) of three shrub species present in the SV of a temperate forest in central Mexico. We collected soil from different sites in the Abies religiosa forest in central Mexico. We collected seeds of Acaena elongata, Ageratina glabrata, and Solanum pubigerum. We used a controlled experimental design with pasteurized soil (-AMF treatments) and unpasteurized soil (+ AMF treatments). We monitored germination percentage, growth (shoot and root weight and total biomass), AMF root colonization, and the mycorrhizal response index (MRI) for each plant species. All three species tested benefited by AMF, showing higher germination rates. Shoot and root weight and total biomass were significantly higher in the + AMF treatment. Solanum pubigerum showed greater stem length and Ageratina glabrata showed greater root development due to AMF. Ageratina glabrata and Acaena elongata were the most responsive to AMF as indicated by MRI. This research underscores the critical role of AMF in the early phenological stages of SV and highlights the potential ecological benefits of AMF in supporting plant germination and plant growth. These results suggest that AMF enhance germination and early growth of secondary vegetation species, which can be considered in management plans for forest ecosystems.