Sequence-related amplified polymorphism (SRAP) markers reveal genetic variation of rice strains obtained by millet DNA injection through coleoptile

IF 1.6 3区农林科学 Q2 AGRONOMY Genetic Resources and Crop Evolution Pub Date : 2024-07-19 DOI:10.1007/s10722-024-02080-6

Yong-Il Choe, Sam-Rang Song, Un-Hyang Ho, Tong-Su Ho, Sung-Jin Sin, Il-Chon Pak, Myong-Bom Choe

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Abstract

Rice represents one of important cereal crops on which half the world’s population depend and it is cultivated on a large scale in Asia. To assist managing rice germplasm, genetic relationship among 48 rice genotypes including MR-4 and MR-11 obtained by coleoptile-mediated millet DNA injection was investigated using sequence-related amplified polymorphism (SRAP) markers. A total of 17 SRAP primer pairs exhibiting reproducible amplification characteristics out of combinations from Me1-Me5, Em1-Em4 were used. Genetic similarity between 48 genotypes revealed by 17 SRAP primer pairs ranged from 0.834 to 0.985. Unweighted pair group method with arithmetic average (UPGMA) tree based on SRAP marker analysis divided 48 rice into two main clusters, that was nearly consistent with classification by growth period. DNA receptor, Sohaechal 16 belonged to second subcluster of Cluster II and MR-4 and MR-11 obtained by introducing millet DNA were assigned to first subcluster of Cluster I. These results suggest that both MR-4 and MR-11 may be considered as distinct germplasm diffferent from DNA receptor Sohaechal 16. Detailed information on genetic relationship of 48 rice genotypes revealed by SRAP markers would contribute to germplasm improvement in the future.

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序列相关扩增多态性（SRAP）标记揭示了通过胚轴注入小米 DNA 获得的水稻品系的遗传变异

水稻是世界上一半人口赖以生存的重要谷类作物之一，在亚洲有大规模种植。为协助管理水稻种质资源，研究人员利用序列相关扩增多态性（SRAP）标记，研究了通过共生孢子介导的小米 DNA 注入获得的 48 个水稻基因型（包括 MR-4 和 MR-11）之间的遗传关系。在 Me1-Me5、Em1-Em4 的组合中，共使用了 17 对 SRAP 引物，这些引物具有可重复的扩增特性。17 对 SRAP 引物揭示的 48 个基因型之间的遗传相似性在 0.834 至 0.985 之间。基于 SRAP 标记分析的算术平均非加权配对组法（UPGMA）树将 48 个水稻分为两大类，与生长期分类基本一致。这些结果表明，MR-4 和 MR-11 可被视为不同于 DNA 受体 Sohaechal 16 的独特种质。SRAP 标记揭示的 48 个水稻基因型遗传关系的详细信息将有助于未来的种质改良。

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来源期刊

Genetic Resources and Crop Evolution 农林科学-农艺学

CiteScore

4.10

自引率

10.00%

发文量

178

审稿时长

3 months

期刊介绍： Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation. Areas of particular interest include: -crop evolution -domestication -crop-weed relationships -related wild species -history of cultivated plants including palaeoethnobotany. Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources. Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany. All contributions are in the English language and are subject to peer reviewing.