Specialization of the Stems into Shoots, Stolons, and Rhizomes in Bermudagrass (Cynodon dactylon L.): Insights from Combined Metabolome and Transcriptome Analyses

IF 3.9 3区 生物学 Q1 PLANT SCIENCES Journal of Plant Growth Regulation Pub Date : 2024-07-18 DOI:10.1007/s00344-024-11420-w
Ziyan Ma, Shuai Yuan, Jingbo Chen, Bing Zhang
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Abstract

As a perennial warm-season turfgrass species with great economic value, bermudagrass (Cynodon dactylon L.) simultaneously has three types of stems: shoot, stolon, and rhizome. However, molecular mechanisms underlying the specialization of the three types of stems remain poorly understood. In this study, the metabolome differences among the three types of stems were analyzed and compared through untargeted metabolomic profiling in combination with transcriptome-wide analyses of the genes participating in the metabolic pathways. A total of 949 metabolites were identified in the three stems, whereas 303, 473, and 330 metabolites were differentially accumulated between shoots and stolons, shoots and rhizomes, and stolons and rhizomes, respectively. Sugars and phenylpropanoids were two enriched categories of metabolites showing preferential accumulation in the three types of stems. Transcriptome and RT-qPCR analyses indicated that gene expression of key enzymes catalyzing the synthesis and transformation of sugars and phenylpropanoids, especially glucose-1-phosphate adenylyltransferase, starch synthase, and phenylalanine ammonia-lyase, were delicately regulated to maintain the sugar-starch and lignin-flavonoid homeostasis in the three stems. The results of this study not only expanded our understanding of metabolism regulation in bermudagrass, but also laid a foundation for molecular mechanism study of stem specialization in this glamorous plant species.

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百慕大草(Cynodon dactylon L.)的茎特化为芽、匍匐茎和根茎:代谢组和转录组联合分析的启示
百慕大草(Cynodon dactylon L.)是一种具有重要经济价值的多年生暖季型草坪草物种,同时具有三种茎:嫩枝、匍匐茎和根茎。然而,人们对这三种茎特化的分子机制仍然知之甚少。本研究通过非靶向代谢组图谱分析,结合参与代谢途径的全转录组基因分析,对三种茎的代谢组差异进行了分析和比较。在三种茎中共鉴定出949种代谢物,而在嫩枝与匍匐茎、嫩枝与根茎以及匍匐茎与根茎之间分别有303种、473种和330种代谢物的积累存在差异。糖类和苯丙酮类是三类茎中优先积累的两类代谢物。转录组和 RT-qPCR 分析表明,催化糖类和苯丙类化合物合成和转化的关键酶,特别是 1-磷酸葡萄糖腺苷基转移酶、淀粉合成酶和苯丙氨酸氨化酶的基因表达受到微妙调控,以维持三种茎中糖-淀粉和木质素-类黄酮的平衡。该研究结果不仅拓展了我们对百慕大草代谢调控的认识,还为这一魅力植物物种茎特化的分子机制研究奠定了基础。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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