Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of biochemistry Pub Date : 2024-11-04 DOI:10.1093/jb/mvae054
Keiko Hirota, Rieko Yamauchi, Mai Miyata, Mariko Kojima, Koichiro Kako, Akiyoshi Fukamizu
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Abstract

The maintenance of germ cells is critical for the prosperity of offspring. The amount of food consumption is known to be closely related to reproduction, i.e. the number of eggs decreases under calorie-restricted conditions in various organisms. Previous studies in Caenorhabditis elegans have reported that calorie restriction reduces the number of eggs and the reduction can be rescued by methionine. However, the effect of methionine on the reproductive process has not been fully understood. In this study, to assess the gonadal function of methionine metabolism, we firstly demonstrated that a depletion in dietary methionine resulted in reduced levels of S-adenosyl-l-methionine (SAM) and S-adenosyl homocysteine in wild-type N2, but not in glp-1 mutants, which possess only a few germ cells. Second, we found no recovery in egg numbers upon methionine administration in SAM synthase (sams)-1 mutants. Furthermore, a reduced number of proliferative zone nuclei exhibited in the sams-1 mutants was not rescued via methionine. Thus, our results have shown that dietary methionine is required for the normal establishment of both the germline progenitor pool and fecundity, mediated by sams-1.

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膳食蛋氨酸通过 sams-1 在秀丽隐杆线虫的增殖区维持和产卵过程中发挥作用。
生殖细胞的维持对后代的繁衍至关重要。众所周知,食物消耗量与生殖密切相关,即在各种生物体内,卡路里限制条件下卵子数量会减少。此前对 elegans(秀丽隐杆线虫)的研究表明,卡路里限制会减少卵的数量,而蛋氨酸可以挽救卵数量的减少。然而,蛋氨酸对生殖过程的影响尚未完全清楚。在本研究中,为了评估蛋氨酸代谢的性腺功能,我们首先证明了膳食蛋氨酸的缺乏会导致野生型 N2 中 S-腺苷-l-蛋氨酸(SAM)和 S-腺苷同型半胱氨酸(SAH)水平的降低,但不会导致仅有少量生殖细胞的 glp-1 突变体中的 S-腺苷-l-蛋氨酸(SAM)和 S-腺苷同型半胱氨酸(SAH)水平的降低。其次,我们发现在 SAM 合成酶(sams)-1 突变体中施用蛋氨酸后,卵子数量没有恢复。此外,sams-1突变体中增殖区细胞核数量的减少也没有通过蛋氨酸得到缓解。因此,我们的研究结果表明,在 Sams-1 的介导下,种系原基库和繁殖力的正常建立都需要膳食蛋氨酸。
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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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