[Expressions of zinc homeostasis proteins, GPR39 and ANO1 mRNA in the sperm of asthenozoospermia patients and their clinical significance].

Q4 Medicine 中华男科学杂志 Pub Date : 2024-01-01

Chun He, Fang-Fang Dai, Jun-Sheng Liu, Ya-Song Geng, Jun-Xia Zhou, Yi-Zhen Hu, Bo Zheng, Shu-Song Wang

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引用次数: 0

Abstract

Objective: To explore the expressions of zinc homeostasis-related proteins, G protein-coupled receptor 39 (GPR39) and ANO1 mRNA in the sperm of patients with asthenozoospermia (AS), and analyze their correlation with sperm motility.

Methods: We collected semen samples from 82 male subjects with PR+NP < 40%, PR < 32% and sperm concentration > 15×10⁶/ml (the AS group, n = 40) or PR+NP ≥ 40%, PR ≥ 32% and sperm concentration > 15×10⁶/ml (the normal control group, n = 42). We analyzed the routine semen parameters and measured the zinc content in the seminal plasma using the computer-assisted sperm analysis system, detected the expressions of zinc transporters (ZIP13, ZIP8 and ZNT10), metallothioneins (MT1G, MT1 and MTF), GPR39, and calcium-dependent chloride channel protein (ANO1) in the sperm by real-time quantitative PCR (RT qPCR), examined free zinc distribution in the sperm by laser confocal microscopy, and determined the expressions of GPR39 and MT1 proteins in the sperm by immunofluorescence staining, followed by Spearman rank correlation analysis of their correlation with semen parameters.

Results: There was no statistically significant difference in the zinc concentration in the seminal plasma between the AS and normal control groups (P>0.05). Compared with the controls, the AS patients showed a significantly reduced free zinc level (P<0.05), relative expressions of MT1G, MTF, ZIP13, GPR39 and ANO1 mRNA (P<0.05), and that of the GPR39 protein in the AS group (P<0.05). No statistically significant differences were observed in the relative expression levels of ZIP8, ZNT10 and MT1 mRNA between the two groups (P>0.05). The relative expression levels of GPR39, ANO1, MT1G and MTF mRNA were positively correlated with sperm motility and the percentage of progressively motile sperm (P<0.05).

Conclusion: The expressions of zinc homeostasis proteins (MT1G, MTF and ZIP13), GPR39 and ANO1 mRNA are downregulated in the sperm of asthenozoospermia patients, and positively correlated with sperm motility.

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[锌平衡蛋白、GPR39 和 ANO1 mRNA 在无精子症患者精子中的表达及其临床意义]。

目的探讨锌平衡相关蛋白、G蛋白偶联受体39（GPR39）和ANO1 mRNA在无精子症（AS）患者精子中的表达，并分析其与精子活力的相关性：我们采集了82名男性受试者的精液样本，这些受试者的PR+NP＜40%、PR＜32%且精子浓度＞15×10⁶/ml（AS组，n=40）或PR+NP≥40%、PR≥32%且精子浓度＞15×10⁶/ml（正常对照组，n=42）。我们分析了精液常规参数，并使用计算机辅助精子分析系统测量了精浆中的锌含量，通过实时定量 PCR（RT qPCR）检测了精子中锌转运体（ZIP13、ZIP8 和 ZNT10）、金属硫蛋白（MT1G、MT1 和 MTF）、GPR39 和钙依赖性氯通道蛋白（ANO1）的表达、通过激光共聚焦显微镜检测精子中游离锌的分布，并通过免疫荧光染色测定精子中 GPR39 和 MT1 蛋白的表达，然后对它们与精液参数的相关性进行斯皮尔曼秩相关分析。结果强直性脊柱炎组与正常对照组精浆中锌浓度差异无统计学意义（P>0.05）。与对照组相比，强直性脊柱炎患者的游离锌水平明显降低（P0.05）。GPR39、ANO1、MT1G和MTF mRNA的相对表达水平与精子活力和渐进活力精子的百分比呈正相关（结论：GPR39、ANO1、MT1G和MTF mRNA的相对表达水平与精子活力和渐进活力精子的百分比呈正相关：锌平衡蛋白（MT1G、MTF和ZIP13）、GPR39和ANO1 mRNA在无精子症患者精子中表达下调，并与精子活力呈正相关。

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来源期刊

中华男科学杂志 Medicine-Medicine (all)

CiteScore

0.40

自引率

0.00%

发文量

5367

期刊介绍： National journal of andrology was founded in June 1995. It is a core journal of andrology and reproductive medicine, published monthly, and is publicly distributed at home and abroad. The main columns include expert talks, monographs (basic research, clinical research, evidence-based medicine, traditional Chinese medicine), reviews, clinical experience exchanges, case reports, etc. Priority is given to various fund-funded projects, especially the 12th Five-Year National Support Plan and the National Natural Science Foundation funded projects. This journal is included in about 20 domestic databases, including the National Science and Technology Paper Statistical Source Journal (China Science and Technology Core Journal), the Source Journal of the China Science Citation Database, the Statistical Source Journal of the China Academic Journal Comprehensive Evaluation Database (CAJCED), the Full-text Collection Journal of the China Journal Full-text Database (CJFD), the Overview of the Chinese Core Journals (2017 Edition), and the Source Journal of the Top Academic Papers of China's Fine Science and Technology Journals (F5000). It has been included in the full text of the American Chemical Abstracts, the American MEDLINE, the American EBSCO, and the database.