Anisotropy component of DTI reveals long-term neuroinflammation following repetitive mild traumatic brain injury in rats.

IF 3.7 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING European Radiology Experimental Pub Date : 2024-07-24 DOI:10.1186/s41747-024-00490-w
Ching Cheng, Chia-Feng Lu, Bao-Yu Hsieh, Shu-Hui Huang, Yu-Chieh Jill Kao
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Abstract

Background: This study aimed to investigate the long-term effects of repetitive mild traumatic brain injury (rmTBI) with varying inter-injury intervals by measuring diffusion tensor metrics, including mean diffusivity (MD), fractional anisotropy (FA), and diffusion magnitude (L) and pure anisotropy (q).

Methods: Eighteen rats were randomly divided into three groups: short-interval rmTBI (n = 6), long-interval rmTBI (n = 6), and sham controls (n = 6). MD, FA, L, and q values were analyzed from longitudinal diffusion tensor imaging at days 50 and 90 after rmTBI. Immunohistochemical staining against neurons, astrocytes, microglia, and myelin was performed. Analysis of variance, Pearson correlation coefficient, and simple linear regression model were used.

Results: At day 50 post-rmTBI, lower cortical FA and q values were shown in the short-interval group (p ≤ 0.038). In contrast, higher FA and q values were shown for the long-interval group (p ≤ 0.039) in the corpus callosum. In the ipsilesional external capsule and internal capsule, no significant changes were found in FA, while lower L and q values were shown in the short-interval group (p ≤ 0.028) at day 90. The q values in the external capsule and internal capsule were negatively correlated with the number of microglial cells and the total number of astroglial cells (p ≤ 0.035).

Conclusion: Tensor scalar measurements, such as L and q values, are sensitive to exacerbated chronic injury induced by rmTBI with shorter inter-injury intervals and reflect long-term astrogliosis induced by the cumulative injury.

Relevance statement: Tensor scalar measurements, including L and q values, are potential DTI metrics for detecting long-term and subtle injury following rmTBI; in particular, q values may be used for quantifying remote white matter (WM) changes following rmTBI.

Key points: The alteration of L and q values was demonstrated after chronic repetitive mild traumatic brain injury. Changing q values were observed in the impact site and remote WM. The lower q values in the remote WM were associated with astrogliosis.

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DTI 的各向异性成分揭示了大鼠重复性轻度脑损伤后的长期神经炎症。
研究背景本研究旨在通过测量弥散张量指标,包括平均弥散率(MD)、分数各向异性(FA)、弥散幅度(L)和纯各向异性(q),研究不同损伤间隔的重复性轻度脑损伤(rmTBI)的长期影响:将 18 只大鼠随机分为三组:短间隔 rmTBI(n = 6)、长间隔 rmTBI(n = 6)和假对照组(n = 6)。对rmTBI后第50天和第90天的纵向弥散张量成像中的MD、FA、L和q值进行分析。对神经元、星形胶质细胞、小胶质细胞和髓鞘进行了免疫组化染色。采用了方差分析、皮尔逊相关系数和简单线性回归模型:结果:在脑损伤后第 50 天,短间隔组的皮质 FA 值和 q 值较低 (p ≤ 0.038)。相比之下,长间隔组胼胝体的 FA 值和 q 值较高(p ≤ 0.039)。在第 90 天,同侧外囊和内囊的 FA 没有发现显著变化,而短间隔组的 L 值和 q 值较低 (p ≤ 0.028)。外囊和内囊的 q 值与小胶质细胞数量和星形胶质细胞总数呈负相关(p ≤ 0.035):张量标度测量,如L值和q值,对损伤间隔较短的rmTBI诱发的慢性损伤加重很敏感,并能反映累积损伤诱发的长期星形胶质细胞病变:张量标度测量,包括 L 值和 q 值,是检测 rmTBI 后长期和细微损伤的潜在 DTI 指标;特别是,q 值可用于量化 rmTBI 后远端白质(WM)的变化:要点:慢性重复性轻度脑损伤后,L 值和 q 值发生了改变。在撞击部位和远端白质中观察到了q值的变化。远端WM中较低的q值与星形胶质细胞增多有关。
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来源期刊
European Radiology Experimental
European Radiology Experimental Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
6.70
自引率
2.60%
发文量
56
审稿时长
18 weeks
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