Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signaling

IF 19.8 1区 医学 Q1 CELL & TISSUE ENGINEERING Cell stem cell Pub Date : 2024-07-23 DOI:10.1016/j.stem.2024.06.015
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Abstract

Embryonic diapause is a reproductive adaptation that enables some mammalian species to halt the otherwise continuous pace of embryonic development. In this dormant state, the embryo exploits poorly understood regulatory mechanisms to preserve its developmental potential for prolonged periods of time. Here, using mouse embryos and single-cell RNA sequencing, we molecularly defined embryonic diapause at single-cell resolution, revealing transcriptional dynamics while the embryo seemingly resides in a state of suspended animation. Additionally, we found that the dormant pluripotent cells rely on integrin receptors to sense their microenvironment and preserve their viability via Yap/Taz-mediated prosurvival signaling.

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以单细胞分辨率分析胚胎休眠揭示了动态转录反应和整合素-Yap/Taz前生存信号的激活
胚胎休眠是一种生殖适应,它使一些哺乳动物物种能够停止胚胎发育的持续步伐。在这种休眠状态下,胚胎利用鲜为人知的调控机制来长时间保持其发育潜能。在这里,我们利用小鼠胚胎和单细胞 RNA 测序,以单细胞分辨率对胚胎休眠进行了分子定义,揭示了胚胎似乎处于休眠状态时的转录动态。此外,我们还发现休眠的多能细胞依赖整合素受体来感知微环境,并通过 Yap/Taz 介导的前生存信号来维持其活力。
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来源期刊
Cell stem cell
Cell stem cell 生物-细胞生物学
CiteScore
37.10
自引率
2.50%
发文量
151
审稿时长
42 days
期刊介绍: Cell Stem Cell is a comprehensive journal covering the entire spectrum of stem cell biology. It encompasses various topics, including embryonic stem cells, pluripotency, germline stem cells, tissue-specific stem cells, differentiation, epigenetics, genomics, cancer stem cells, stem cell niches, disease models, nuclear transfer technology, bioengineering, drug discovery, in vivo imaging, therapeutic applications, regenerative medicine, clinical insights, research policies, ethical considerations, and technical innovations. The journal welcomes studies from any model system providing insights into stem cell biology, with a focus on human stem cells. It publishes research reports of significant importance, along with review and analysis articles covering diverse aspects of stem cell research.
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