A stepwise multi-stage continuous dielectrophoresis separation microfluidic chip with microfilter structures

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2024-07-19 DOI:10.1016/j.talanta.2024.126585

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Abstract

The separation of target microparticles using microfluidic systems owns extensive applications in biomedical, chemical, and materials science fields. Integration of microfluidic sorting systems employing dielectrophoresis (DEP) technology has been widely investigated. However, enhancing separation efficiency, purity, stability, and integration remains a pressing issue. This study proposes a stepwise multi-stage continuous DEP separation microfluidic chip with a microfilter structure. By leveraging a stepwise electrode configuration, a gradient electric field is generated to drive target microparticles along the electric field gradient, thereby enhancing separation efficiency. Innovative integration of a microfilter structure facilitates simultaneous filtration and improves flow field distribution, thus enhancing system stability. Through the synergistic effect of stepwise electrodes and the microfilter structure, superior coupling of electric and flow fields is achieved, consequently improving the sorting purity, separation efficiency, and system stability of the DEP-based microfluidic sorting system. Validation through simulation and separation of polystyrene microspheres demonstrates the excellent particle separation performance of the proposed system. It evidently shows potential for seamless extension to various biological microparticle sorting applications, harboring significant prospects in the biomedical domain field.

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具有微过滤器结构的分步多级连续介电泳分离微流体芯片。

利用微流体系统分离目标微粒在生物医学、化学和材料科学领域有着广泛的应用。采用介电泳（DEP）技术的微流体分拣系统集成已得到广泛研究。然而，如何提高分离效率、纯度、稳定性和集成度仍是一个亟待解决的问题。本研究提出了一种具有微过滤器结构的分步多级连续 DEP 分离微流控芯片。利用分步式电极配置，产生梯度电场，驱动目标微颗粒沿电场梯度运动，从而提高分离效率。微滤器结构的创新整合有利于同时进行过滤，并改善了流场分布，从而提高了系统的稳定性。通过阶跃电极和微过滤器结构的协同作用，实现了电场和流场的良好耦合，从而提高了基于 DEP 的微流控分拣系统的分拣纯度、分离效率和系统稳定性。通过对聚苯乙烯微球的模拟和分离进行验证，证明了所提出的系统具有出色的颗粒分离性能。显然，该系统具有无缝扩展到各种生物微颗粒分拣应用的潜力，在生物医学领域前景广阔。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.