Citicoline modulates inflammatory signaling pathways in the spleen of rats exposed to gamma-radiation.

IF 2.9 4区医学 Q3 IMMUNOLOGY Immunopharmacology and Immunotoxicology Pub Date : 2024-08-01 Epub Date: 2024-07-25 DOI:10.1080/08923973.2024.2381759

Nahed Abdel-Aziz, Mostafa Saif-Elnasr

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Abstract

Background and aim: The spleen has an essential role in immune responses regulation and is considered the biggest peripheral immune organ. Citicoline is used for various brain disorders management. This study aimed to examine the using possibility of citicoline to treat γ-radiation-induced splenic inflammation in rats.

Materials and methods: Eighteen male albino rats were classified into: Group 1 (control) animals were kept as control. Group 2 (γ-radiation) animals were total-body γ-irradiated with 6 Gy. Group 3 (γ-radiation + citicoline) rats were γ-irradiated with 6 Gy, then injected intraperitoneally with citicoline (300 mg/kg/d) 5 min after irradiation for one week. Levels of TNF-α, IL-1β, iNOS, NF-κB, JAK2, and STAT3 were determined in spleen tissue, along with histopathological examination.

Results: Rats exposure to gamma-radiation led to elevation in splenic TNF-α, IL-1β, NF-κB, iNOS, JAK2, and STAT3 levels significantly. Treatment with citicoline after gamma-radiation exposure improved this elevation, and modulated gamma-radiation-induced histopathological alterations.

Conclusions: This data showed that citicoline inhibited γ-radiation-induced splenic inflammation via suppressing NF-κB and JAK2/STAT3 signaling pathways in spleen tissue.

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胞二磷胆碱调节暴露于伽马射线的大鼠脾脏中的炎症信号通路

背景和目的：脾脏在免疫反应调节中起着至关重要的作用，被认为是最大的外周免疫器官。柠檬胆碱可用于治疗各种脑部疾病。本研究旨在探讨使用柠檬黄素治疗γ射线诱导的大鼠脾脏炎症的可能性：将 18 只雄性白化大鼠分为两组：第 1 组（对照组）作为对照。第 2 组（γ 辐射）动物全身接受 6 Gy γ 辐射。第 3 组（γ-辐照 + 胞二磷胆碱）大鼠接受 6 Gy γ-辐照，然后在辐照后 5 分钟腹腔注射胞二磷胆碱（300 mg/kg/d），持续一周。测定脾组织中 TNF-α、IL-1β、iNOS、NF-κB、JAK2 和 STAT3 的水平，并进行组织病理学检查：结果：大鼠接受伽马射线照射后，脾脏TNF-α、IL-1β、NF-κB、iNOS、JAK2和STAT3水平显著升高。在伽马射线照射后使用柠檬胆碱治疗可改善这种升高，并调节伽马射线诱导的组织病理学改变：这些数据表明，柠檬胆碱通过抑制脾组织中的 NF-κB 和 JAK2/STAT3 信号通路，抑制了γ-射线诱导的脾脏炎症。

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来源期刊

Immunopharmacology and Immunotoxicology 医学-毒理学

CiteScore

5.40

自引率

0.00%

发文量

133

审稿时长

4-8 weeks

期刊介绍： The journal Immunopharmacology and Immunotoxicology is devoted to pre-clinical and clinical drug discovery and development targeting the immune system. Research related to the immunoregulatory effects of various compounds, including small-molecule drugs and biologics, on immunocompetent cells and immune responses, as well as the immunotoxicity exerted by xenobiotics and drugs. Only research that describe the mechanisms of specific compounds (not extracts) is of interest to the journal. The journal will prioritise preclinical and clinical studies on immunotherapy of disorders such as chronic inflammation, allergy, autoimmunity, cancer etc. The effects of small-drugs, vaccines and biologics against central immunological targets as well as cell-based therapy, including dendritic cell therapy, T cell adoptive transfer and stem cell therapy, are topics of particular interest. Publications pointing towards potential new drug targets within the immune system or novel technology for immunopharmacological drug development are also welcome. With an immunoscience focus on drug development, immunotherapy and toxicology, the journal will cover areas such as infection, allergy, inflammation, tumor immunology, degenerative disorders, immunodeficiencies, neurology, atherosclerosis and more. Immunopharmacology and Immunotoxicology will accept original manuscripts, brief communications, commentaries, mini-reviews, reviews, clinical trials and clinical cases, on the condition that the results reported are based on original, clinical, or basic research that has not been published elsewhere in any journal in any language (except in abstract form relating to paper communicated to scientific meetings and symposiums).