Magnetic fibrin nanofiber hydrogel delivering iron oxide magnetic nanoparticles promotes peripheral nerve regeneration.

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2024-06-20 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae075
Juncong Hong, Dongze Wu, Haitao Wang, Zhe Gong, Xinxin Zhu, Fang Chen, Zihang Wang, Mingchen Zhang, Xiumei Wang, Xiangqian Fang, Shuhui Yang, Jinjin Zhu
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Abstract

Peripheral nerve injury is a debilitating condition that have a profound impact on the overall quality of an individual's life. The repair of peripheral nerve defects continues to present significant challenges in the field. Iron oxide magnetic nanoparticles (IONPs) have been recognized as potent nanotools for promoting the regeneration of peripheral nerves due to their capability as biological carriers and their ability to template the hydrogel structure under an external magnetic field. This research used a fibrin nanofiber hydrogel loaded with IONPs (IONPs/fibrin) to promote the regeneration of peripheral nerves in rats. In vitro examination of PC12 cells on various concentrations of IONPs/fibrin hydrogels revealed a remarkable increase in NGF and VEGF expression at 2% IONPs concentration. The biocompatibility and degradation of 2% IONPs/fibrin hydrogel were assessed using the in vivo imaging system, demonstrating subcutaneous degradation within a week without immediate inflammation. Bridging a 10-mm sciatic nerve gap in Sprague Dawley rats with 2% IONPs/fibrin hydrogel led to satisfactory morphological recovery of myelinated nerve fibers. And motor functional recovery in the 2% IONPs/fibrin group was comparable to autografts at 6, 9 and 12 weeks postoperatively. Hence, the composite fibrin hydrogel incorporating 2% IONPs exhibits potential for peripheral nerve regeneration.

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输送氧化铁磁性纳米颗粒的磁性纤维蛋白纳米纤维水凝胶可促进外周神经再生。
周围神经损伤是一种使人衰弱的疾病,对个人的整体生活质量有着深远的影响。外周神经损伤的修复仍然是该领域的重大挑战。氧化铁磁性纳米粒子(IONPs)被认为是促进周围神经再生的有效纳米工具,因为它们具有生物载体的能力,并能在外部磁场下模板化水凝胶结构。本研究使用负载 IONPs 的纤维蛋白纳米纤维水凝胶(IONPs/纤维蛋白)来促进大鼠周围神经的再生。对PC12细胞在不同浓度的IONPs/纤维蛋白水凝胶上的体外检测显示,当IONPs浓度为2%时,NGF和VEGF的表达量显著增加。使用体内成像系统对 2% IONPs/纤维蛋白水凝胶的生物相容性和降解情况进行了评估,结果表明这种水凝胶在一周内可在皮下降解,且不会立即引起炎症。用2% IONPs/纤维蛋白水凝胶桥接Sprague Dawley大鼠10毫米的坐骨神经间隙,髓鞘神经纤维的形态恢复令人满意。术后6周、9周和12周,2% IONPs/纤维蛋白组的运动功能恢复与自体移植相当。因此,含有2% IONPs的复合纤维蛋白水凝胶具有周围神经再生的潜力。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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