Genetic characterisation of lymphogranuloma venereum in Spain: a multicentre study.

IF 3.6 3区医学 Q2 INFECTIOUS DISEASES Sexually Transmitted Infections Pub Date : 2024-07-24 DOI:10.1136/sextrans-2023-056021

Paula Salmerón, Judit Serra-Pladevall, Martí Vall-Mayans, Laura Villa, Luis Otero-Guerra, Ana Milagro, María D Maciá, Samuel Bernal, Luis Piñeiro

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Abstract

Introduction: Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis genotypes L1-L3. A combination of techniques with high discriminatory capacity such as multilocus sequence typing (MLST) and the analysis of the ompA gene may be useful to determine the greater penetration of certain strains in transmission networks and their relationship with certain tropisms.

Aim: The aim of this study was to investigate the molecular epidemiology of LGV isolates from different regions of Spain.

Methods: Genetic characterisation of LGV isolates detected in six hospitals from Spain between 2018 and 2019 was performed. MLST (five variable regions: hctB, CT058, CT144, CT172 and pbpB) and ompA sequence determination were used to study the LGV strains.

Results: Most of the 161 LGV isolates (93.8%) were detected in men who have sex with men (MSM). At least 43.5% of the patients presented with HIV coinfection and 53.4% were symptomatic, with proctitis being the most prevalent symptom (73.3%). Most isolates were detected in Barcelona (n=129).The distribution of ompA genovariants was as follows: 56.1% belonged to L2, 24.3% to L2b, 5.4% to L2bV1, 4.7% to L2bV4, 4.1% to L1, 2.7% to L2b/D-Da, 2.0% to L2bV2 and 0.7% to L2bV7. MLST was successfully performed in 81 samples and 9 different sequence types (STs) were detected. The ompA and MLST combination obtained 17 different genetic profiles, with L2-ST53 and L2-ST58 being the most prevalent (29.5% and 14.1%, respectively). L1 genotype strains belonged to ST23 (n=3) and ST2 (n=3).

Conclusion: LGV infections were mainly found in MSM living with HIV and with proctitis. The joint analysis of ompA and MLST genetic characterisation techniques showed a high discriminatory capacity. Our findings suggest a cocirculation of L2 and L2b ompA genotypes, and with the inclusion of MLST characterisation, the most prevalent profiles were ompA genotype L2-MLST ST53 and L2-MLST ST58.

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西班牙淋巴肉芽肿的遗传特征：一项多中心研究。

导言：淋巴肉芽肿（LGV）是由沙眼衣原体基因型 L1-L3 引起的性传播感染。将多焦点序列分型（MLST）和 ompA 基因分析等具有高度鉴别能力的技术结合起来，可能有助于确定某些菌株在传播网络中的渗透力以及它们与某些滋养体之间的关系：对2018年至2019年期间在西班牙6家医院检测到的LGV分离株进行了遗传特征分析。采用MLST（五个可变区：hctB、CT058、CT144、CT172和pbpB）和ombA序列测定来研究LGV菌株：在 161 株 LGV 分离物中，大部分（93.8%）在男男性行为者（MSM）中检出。至少 43.5%的患者合并感染艾滋病毒，53.4%的患者有症状，直肠炎是最常见的症状（73.3%）。大多数分离株在巴塞罗那检测到（n=129）：56.1%属于L2，24.3%属于L2b，5.4%属于L2bV1，4.7%属于L2bV4，4.1%属于L1，2.7%属于L2b/D-Da，2.0%属于L2bV2，0.7%属于L2bV7。在 81 个样本中成功进行了 MLST，检测到 9 种不同的序列类型（ST）。ompA 和 MLST 组合得到了 17 种不同的基因型，其中 L2-ST53 和 L2-ST58 最为普遍（分别占 29.5%和 14.1%）。L1基因型菌株属于ST23（3株）和ST2（3株）：结论：LGV感染主要出现在感染艾滋病毒和患有直肠炎的男男性行为者中。ombA和MLST基因特征技术的联合分析显示了很高的鉴别能力。我们的研究结果表明，L2 和 L2b ompA 基因型存在共循环现象，在纳入 MLST 特征后，最普遍的特征是 ompA 基因型 L2-MLST ST53 和 L2-MLST ST58。

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来源期刊

Sexually Transmitted Infections 医学-传染病学

CiteScore

5.70

自引率

8.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Sexually Transmitted Infections is the world’s longest running international journal on sexual health. It aims to keep practitioners, trainees and researchers up to date in the prevention, diagnosis and treatment of all STIs and HIV. The journal publishes original research, descriptive epidemiology, evidence-based reviews and comment on the clinical, public health, sociological and laboratory aspects of sexual health from around the world. We also publish educational articles, letters and other material of interest to readers, along with podcasts and other online material. STI provides a high quality editorial service from submission to publication.