Epidemiological pattern and genomic insights into multidrug-resistant ST491 Acinetobacter baumannii BD20 isolated from an infected wound in Bangladesh: Concerning co-occurrence of three classes of beta lactamase genes

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-07-25 DOI:10.1016/j.jgar.2024.07.009
Israt Islam , Badriya Mubashshira , Spencer Mark Mondol , Otun Saha , M. Shaminur Rahman , Afroza Khan , Amiruzzaman , Md. Mizanur Rahaman
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Abstract

Objective

Multidrug-resistant (MDR) Acinetobacter baumannii is a major issue within healthcare facilities in Bangladesh due to its frequent association with hospital-acquired infections. In this study we report on a carbapenem-resistant draft genome sequence of an A. baumannii BD20 sample isolated from an infected wound in Bangladesh.

Methods

A. baumannii BD20 was isolated from an infected burn wound. Whole-genome sequencing was carried out and annotated using PGAP and Prokka. Sequence type, antimicrobial resistance genes, virulence factor genes, and metal resistance genes were investigated. Core genome multilocus sequence typing–based phylogenomic analysis between A. baumannii BD20 and 213 A. baumannii strains retrieved from the NCBI GenBank database was performed using the BacWGSTdb 2.0 server.

Results

A. baumannii BD20 (MLST 491) was resistant to all the antibiotics tested, except for colistin and polymyxin B. Along with many other antibiotic resistance genes, the isolate harbored three classes of beta lactamase–producing genes: blaGES-11 (class A), blaOXA-69 (class D), blaADC-10 (class C), and blaADC-11 (class C). Additionally, the strain carried several virulence genes and metal resistance determinants, which may contribute to its increased virulence. Core genome MLST–based phylogenomic analysis revealed that A. baumannii BD20 was closely related to another ST491 strain isolated from Singapore.

Conclusions

The findings of this study underscore the growing challenge of MDR A. baumannii, emphasizing the need for vigilant surveillance and infection-control measures in healthcare settings in order to address these emerging threats effectively.

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从孟加拉国感染伤口中分离出的耐多药 ST 491 鲍曼不动杆菌 BD20 的流行病学模式和基因组学见解:关于三类β内酰胺酶基因的共存。
目的:耐多药(MDR)鲍曼不动杆菌(A. baumannii)是孟加拉国医疗机构中的一个主要问题,因为它经常与医院感染有关。在本研究中,我们报告了从孟加拉国感染伤口中分离出的耐碳青霉烯类鲍曼不动杆菌 BD20 的基因组序列草案:方法:从感染的烧伤伤口中分离出鲍曼不动杆菌 BD20。进行了全基因组测序,并用 PGAP 和 Prokka 进行了注释。研究了序列类型、抗菌药耐药基因、毒力因子基因和金属耐药基因。使用 BacWGSTdb 2.0 服务器对鲍曼不动杆菌 BD20 和从 NCBI GenBank 数据库中检索到的 213 株鲍曼不动杆菌进行了基于核心基因组 MLST(cgMLST)的系统进化分析:除许多其他抗生素耐药基因外,该分离株还携带有三种产β内酰胺酶的基因:blaGES-11(A类)、blaOXA-69(D类)、blaADC-10(C类)和blaADC-11(C类)。此外,该菌株还携带多个毒力基因和金属抗性决定簇,这可能是其毒力增强的原因。基于 cgMLST 的系统发生组分析表明,鲍曼不动杆菌 BD20 与另一株从新加坡分离的 ST491 菌株关系密切:本研究的结果凸显了多重耐药鲍曼不动杆菌所带来的日益严峻的挑战,强调了在医疗环境中采取警惕性监测和感染控制措施的必要性,以有效应对这些新出现的威胁。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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