Ameliorative effects of sinapic acid against vancomycin-induced testicular oxidative damage, apoptosis, inflammation, testicular histopathologic disorders and decreased epididymal sperm quality

IF 3.3 4区 医学 Q2 REPRODUCTIVE BIOLOGY Reproductive toxicology Pub Date : 2024-07-25 DOI:10.1016/j.reprotox.2024.108666
Serkan Ali Akarsu , Mustafa İleritürk , Sefa Küçükler , Nurhan Akaras , Cihan Gür , Fatih Mehmet Kandemir
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Abstract

In this study, it was aimed to determine the effect of sinapic acid (SNP), a polyphenol with antioxidant, anti-inflammatory and antibacterial properties, on testicular damage caused by vancomycin (VCM), a widely used antibiotic against gram positive bacteria. A total of 35 male Sprague Dawley rats were used in the study, divided into five groups: control, VCM, SNP, VCM + SNP 10, and VCM + SNP 20. Following a week of oral administration, the rats were euthanized under sevoflurane anesthesia. While the VCM group had a significant increase in MDA levels, the SNP administration inhibited the increase in MDA levels. VCM led to a significant decrease in GSH levels, SOD, CAT, and GPx activity in the testicular tissue of rats, while SNP administration increased these antioxidant levels. SNP administration decreased the mRNA expression levels of VCM induced Nrf-2, HO-1, and NQO1 in testicular tissue while increasing the levels of MAPK14, MAPK15, JNK, P53, Apaf-1, Caspase-3, Caspase-6, Caspase-9, and Beclin-1 mRNA transcript levels. The VCM group showed a significant increase in Bax and NF-κB levels in testicular tissue, while Bcl-2 levels decreased. VCM significantly decreased sperm motility and increased the percentage of damaged sperm in rats. Histopathological results revealed that VCM caused disruption of basement membranes and disorganization of seminiferous tubules, but SNP administration preserved testicular histology. As a result, VCM increased oxidative stress, apoptosis, and autophagy in the testicular tissue of rats, altered testicular histopathology, and decreased sperm quality, while SNP decreased these effects.

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山奈酸对万古霉素引起的睾丸氧化损伤、细胞凋亡、炎症、睾丸组织病理学紊乱和附睾精子质量下降有改善作用。
本研究旨在确定具有抗氧化、抗炎和抗菌特性的多酚类化合物西那吡酸(SNP)对万古霉素(VCM)(一种广泛用于抗革兰氏阳性菌的抗生素)引起的睾丸损伤的影响。研究共使用了 35 只雄性 Sprague Dawley 大鼠,分为五组:对照组、VCM 组、SNP 组、VCM + SNP 10 组和 VCM + SNP 20 组。大鼠口服一周后,在七氟醚麻醉下安乐死。单体氯乙烯组的 MDA 含量显著增加,而 SNP 组则抑制了 MDA 含量的增加。氯乙烯单体会导致大鼠睾丸组织中 GSH 水平、SOD、CAT 和 GPx 活性显著下降,而施用 SNP 则会提高这些抗氧化剂的水平。施用 SNP 会降低 VCM 诱导的睾丸组织中 Nrf-2、HO-1 和 NQO1 的 mRNA 表达水平,同时提高 MAPK14、MAPK15、JNK、P53、Apaf-1、Caspase-3、Caspase-6、Caspase-9 和 Beclin-1 mRNA 转录水平。VCM 组睾丸组织中的 Bax 和 NF-κB 水平明显升高,而 Bcl-2 水平下降。VCM 明显降低了大鼠的精子活力,增加了受损精子的比例。组织病理学结果表明,VCM 会导致基底膜破坏和曲细精管紊乱,而 SNP 则可保留睾丸组织学。因此,VCM 增加了大鼠睾丸组织中的氧化应激、细胞凋亡和自噬,改变了睾丸组织病理学,降低了精子质量,而 SNP 则减少了这些影响。
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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