Combined Application of 2,4-Epibrassinolide and K2SO4 Improved the Cadmium Tolerance in Tobaccos by Protecting Chloroplast and Reducing Cd Accumulation

IF 3.9 3区 生物学 Q1 PLANT SCIENCES Journal of Plant Growth Regulation Pub Date : 2024-07-24 DOI:10.1007/s00344-024-11427-3
Yalin Yang, Lijun Qin, Piao Wu, Xiangnan Qin, Yaxuan Xiang, Xian Gong
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Abstract

Potassium sulfate (K2SO4) or 2,4-Epibrassinolide (EBL) mediated response to cadmium (Cd) stress in plants has been widely reported, but the joint effect both on plants in response to Cd stress remains obscure. Herein, our results showed the combined application of K2SO4 (10 mM) and EBL (0.2 μM) on tobacco plants under Cd stress (CdCl2, 100 μM) relieved Cd toxicity by improving the activities of AOEs and reducing the contents of decreasing superoxide (H2O2 and O2) and cadmium ion. As a result, the synergetic application of K2SO4 and EBL protected the integrity of chloroplast, which ensured a normal process of photosynthesis without a significant decrease in photosynthetic pigment content. Additionally, the combined application of K2SO4 and EBL also inhibited the expression of the Cd transport-related gene NtNramp1 and enhanced the Cd efflux-related gene NtHMA2. Moreover, the combined treatment of EBL associated with K2SO4 also effectively reduced the Cd accumulation in tobacco leaves under Cd stress compared with the groups treated separately, which implied a potentially synergistic role of EBL and K2SO4 in alleviating Cd toxicity in tobacco plants. This study provides a theoretical reference for the further analysis of the molecular mechanism of K2SO4 and EBL in jointly mediating the response to heavy metals in plants.

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2,4-表紫苏内酯和 K2SO4 的联合应用通过保护叶绿体和减少镉积累提高烟草对镉的耐受性
硫酸钾(K2SO4)或2,4-表紫草素内酯(EBL)介导的植物对镉(Cd)胁迫的响应已被广泛报道,但二者对植物响应镉胁迫的联合效应仍不清楚。在此,我们的研究结果表明,K2SO4(10 mM)和 EBL(0.2 μM)联合应用于镉胁迫(CdCl2,100 μM)下的烟草植株,通过提高 AOEs 的活性、降低超氧化物(H2O2 和 O2-)和镉离子的含量,缓解了镉的毒性。因此,K2SO4 和 EBL 的协同作用保护了叶绿体的完整性,从而确保了光合作用的正常进行,且光合色素含量没有显著下降。此外,联合施用 K2SO4 和 EBL 还抑制了镉转运相关基因 NtNramp1 的表达,增强了镉外排相关基因 NtHMA2 的表达。此外,与单独处理组相比,EBL与K2SO4联合处理还能有效降低镉胁迫下烟草叶片的镉积累,这意味着EBL和K2SO4在缓解烟草植物镉毒性方面可能具有协同作用。该研究为进一步分析 K2SO4 和 EBL 共同介导植物对重金属反应的分子机制提供了理论参考。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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