Pre-column Derivative HPLC and LC–Orbitrap-MS Analysis of Monosaccharides and Non-polysaccharides in Polygonati Rhizoma

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2024-07-23 DOI:10.1007/s10337-024-04350-y

Ling Liang, Yu Li, Caiyun Peng, Luyun Ning, Gangqiang Yi, Wei Wang, Hanwen Yuan, Pingan Liu

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Abstract

Polygonati Rhizoma, as a traditional medicinal herb, possesses pharmacological effects enhancing physical strength and immunity. In this study, a systematic analysis of the monosaccharide and non-polysaccharides components in Polygonati Rhizoma was conducted using pre-column derivatization high-performance liquid chromatography (HPLC–DAD) and liquid chromatography coupled to electrostatic orbitrap high-resolution mass spectrometry (LC–Orbitrap-MS) techniques. The polysaccharides from Polygonati Rhizoma were initially extracted, hydrolyzed, and derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP), resulting in the successful detection of five monosaccharides. The high sensitivity and specificity of the HPLC–DAD method were confirmed. Furthermore, by comparing the external standard method (ESM) and the quantitative analysis of multi-components by single-marker (QAMS) revealed that D-mannose is the most abundant monosaccharide in Polygonati Rhizoma. The LC–Orbitrap-MS analysis of Polygonati Rhizoma led to the identification of 53 compounds, including organic acids, amino acids, amides, saponins, alkaloids, esters, and others. This research provided significant data for the chemical composition analysis and the pharmacological basis study of Polygonati Rhizoma.

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柱前衍生 HPLC 和 LC-Orbitrap-MS 分析黄精中的单糖和非多糖类物质

黄精是一种传统药材，具有增强体力和免疫力的药理作用。本研究采用柱前衍生化高效液相色谱法（HPLC-DAD）和液相色谱-静电轨道阱高分辨质谱法（LC-Orbitrap-MS）对黄精中的单糖和非多糖成分进行了系统分析。首先对黄精中的多糖进行提取、水解并用 1-苯基-3-甲基-5-吡唑啉酮（PMP）进行衍生化，成功地检测出 5 种单糖。HPLC-DAD 方法的高灵敏度和特异性得到了证实。此外，通过比较外标法（ESM）和单标记多组分定量分析法（QAMS），发现D-甘露糖是黄精中含量最高的单糖。通过对黄精的 LC-Orbitrap-MS 分析，鉴定出 53 种化合物，包括有机酸、氨基酸、酰胺、皂甙、生物碱、酯类等。该研究为黄精的化学成分分析和药理基础研究提供了重要数据。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.