Comparative Population Biology and Related Gene Expression in the Beta-Cypermethrin-Resistant Strains of Bactrocera Dorsalis (Hendel)

IF 2.7 2区农林科学 Q1 ENTOMOLOGY Insects Pub Date : 2024-07-26 DOI:10.3390/insects15080569

Doudou Li, Langjie Chen, Xinyan Cai, Yixiang Qi, Yongyue Lu

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Abstract

Diptera and Lepidoptera species have the highest levels of insecticide resistance, and the mechanism of drug resistance has been studied in detoxification metabolism genes such as P450, GST, EST, and ABC. Since Bactrocera dorsalis are resistant to a variety of chemicals, the pattern and mechanism of resistance in Bactrocera dorsalis have been investigated from a variety of aspects such as detoxification metabolism genes, detoxification enzymes, intestinal symbiotic bacteria, and synergists in the world. In this study, 51 species and 149 detoxification metabolism genes were annotated in the Suppression Subtractive Hybridization (SSH) library, and 12 candidate genes related to beta-cypermethrin resistance were screened and quantitatively expressed in this library. Two genes were found to be upregulated in the egg stage, three genes in the larval stage, one gene in the pupal stage, and five genes in the adult stage, and four genes were found to be upregulated in the midgut and the malacca ducts in the midgut. The expression of cyp6g1, cyp6a22, GST-Epsilon9, and Trypsin-4 genes was upregulated in resistant strains, with the most obvious upregulation occurring in the midgut and the Malpighian tubules. These results provide new insights into the study of pesticide resistance in quarantine insects.

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耐乙型氯氰菊酯菌株的种群生物学比较和相关基因表达

双翅目和鳞翅目物种对杀虫剂的抗药性水平最高，抗药性机制的研究涉及 P450、GST、EST 和 ABC 等解毒代谢基因。由于背甲线虫对多种化学物质具有抗药性，世界各国从解毒代谢基因、解毒酶、肠道共生菌、增效剂等多方面研究了背甲线虫的抗药性模式和机制。本研究在抑制减弱杂交（SSH）文库中注释了51个物种和149个解毒代谢基因，筛选出12个与β-氯氰菊酯抗性相关的候选基因，并在该文库中进行了定量表达。发现卵期有 2 个基因上调，幼虫期有 3 个基因上调，蛹期有 1 个基因上调，成虫期有 5 个基因上调，中肠和中肠马六甲管有 4 个基因上调。在抗性品系中，cyp6g1、cyp6a22、GST-Epsilon9和胰蛋白酶-4基因的表达上调，其中以中肠和马氏管的上调最为明显。这些结果为研究检疫昆虫的抗药性提供了新的视角。

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来源期刊

Insects Agricultural and Biological Sciences-Insect Science

CiteScore

5.10

自引率

10.00%

发文量

1013

审稿时长

21.77 days

期刊介绍： Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.