A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2024-07-25 DOI:10.1016/j.talanta.2024.126597

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Abstract

As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO⁻) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO⁻ in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, SJD was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe Nano-SJD. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO⁻ in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO⁻ in real-water samples. Additionally, the robust fluorescent imaging behavior of Nano-SJD for visualization of ClO⁻ in living cells highlights its broad potential in biosystem applicability.

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用于快速检测纯水中次氯酸盐的自组装纳米探针及其在活细胞、食品和环境系统中的应用。

作为参与各种生理和病理过程的重要 ROS 物种，高浓度的次氯酸盐（ClO-）会对健康和安全造成严重影响，因此需要高效的检测方法。本研究介绍了一种水溶性荧光纳米探针 Nano-SJD，可有效检测食品样品和活细胞中的 ClO-。以 N, N-二甲基硫代氨基甲酰（DMTC）为识别基团的小分子探针 SJD 是在萘衍生物的基础上构建的。为了进一步提高水溶性，SJD 与一种两亲共聚物（mPEG-DSPE）组装在一起，制备出一种水溶性荧光纳米探针 Nano-SJD。幸运的是，该纳米探针保留了小分子的优良特性，对水溶液中的 ClO- 具有非常好的光学响应，具有超快速响应（1 秒内）、干扰小、检测限低（0.39 μM）和良好的 pH 稳定性等优点。更重要的是，我们还开发出了与智能手机兼容的试纸条，可方便地现场检测真实水样中的 ClO-。此外，Nano-SJD 在活细胞中可视化 ClO- 的强大荧光成像性能也凸显了其在生物系统中的广泛应用潜力。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.