The Characterization and Regulation of Schwann Cells in the Tooth Germ Development and Odontogenic Differentiation.

IF 2.5 4区 医学 Q3 CELL & TISSUE ENGINEERING International journal of stem cells Pub Date : 2024-11-30 Epub Date: 2024-07-29 DOI:10.15283/ijsc23205
Jing He, Ting Wang, Danyang Liu, Jun Yang, Yuanpei He, Shouliang Zhao, Yanqin Ju
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Abstract

Schwann cells (SCs), a type of glial cell in the peripheral nervous system, can serve as a source of mesenchymal stem cells (MSCs) to repair injured pulp. This study aimed to investigate the role of SCs in tooth germ development and repair of pulp injury. We performed RNA-seq and immunofluorescent staining on tooth germs at different developmental stages. The effect of L-type calcium channel (LTCC) blocker nimodipine on SCs odontogenic differentiation was analyzed by real-time polymerase chain reaction and Alizarin Red S staining. We used the PLP1-CreERT2/ Rosa26-GFP tracing mice model to examine the role of SCs and Cav1.2 in self-repair after pulp injury. SC-specific markers expressed in rat tooth germs at different developmental stages. Nimodipine treatment enhanced mRNA levels of osteogenic markers (DSPP, DMP1, and Runx2) but decreased calcium nodule formation. SCs-derived cells increased following pulp injury and Cav1.2 showed a similar response pattern as SCs. The different SCs phenotypes are coordinated in the whole process to ensure tooth development. Blocking the LTCC with nimodipine promoted SCs odontogenic differentiation. Moreover, SCs participate in the process of injured dental pulp repair as a source of MSCs, and Cav1.2 may regulate this process.

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雪旺细胞在牙胚发育和牙源性分化过程中的特征和调控。
许旺细胞(SCs)是周围神经系统中的一种神经胶质细胞,可作为间充质干细胞(MSCs)的来源修复损伤的牙髓。本研究旨在探讨SCs在牙胚发育和牙髓损伤修复中的作用。我们对不同发育阶段的牙胚进行了RNA-seq和免疫荧光染色。通过实时聚合酶链反应和茜素红S染色分析了L型钙通道(LTCC)阻滞剂尼莫地平对SCs牙胚分化的影响。我们利用PLP1-CreERT2/ Rosa26-GFP追踪小鼠模型研究了SCs和Cav1.2在牙髓损伤后自我修复中的作用。大鼠牙胚在不同发育阶段表达的SC特异性标记。尼莫地平治疗可提高成骨标志物(DSPP、DMP1 和 Runx2)的 mRNA 水平,但会减少钙结节的形成。牙髓损伤后,SCs衍生细胞增加,Cav1.2表现出与SCs相似的反应模式。不同的 SCs 表型在确保牙齿发育的整个过程中相互协调。用尼莫地平阻断LTCC可促进SCs牙本质分化。此外,SCs作为间充质干细胞的来源参与了损伤牙髓的修复过程,而Cav1.2可能调控这一过程。
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来源期刊
International journal of stem cells
International journal of stem cells Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.10
自引率
4.30%
发文量
38
期刊介绍: International Journal of Stem Cells (Int J Stem Cells), a peer-reviewed open access journal, principally aims to provide a forum for investigators in the field of stem cell biology to present their research findings and share their visions and opinions. Int J Stem Cells covers all aspects of stem cell biology including basic, clinical and translational research on genetics, biochemistry, and physiology of various types of stem cells including embryonic, adult and induced stem cells. Reports on epigenetics, genomics, proteomics, metabolomics of stem cells are welcome as well. Int J Stem Cells also publishes review articles, technical reports and treatise on ethical issues.
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