Ibuprofen as an adjuvant to conventional antimicrobials and essential oil compounds against skin pathogens.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-08-05 DOI:10.1093/jambio/lxae186

Shivar Simbu, Ané Orchard, Maryna van de Venter, Sandy van Vuuren

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Abstract

Aims: Antimicrobial resistance continues to be a growing concern, resulting in increased use of drug combinations. Antibiotic adjuvants are an emerging strategy that may potentiate an antibiotics efficacy. Ibuprofen's polypharmacological properties have been investigated for their antimicrobial and host-modulating potential. This study aimed to investigate the potential of a novel multidrug combination involving ibuprofen, essential oil compounds (EOCs), and conventional antimicrobials against skin pathogens.

Methods and results: The minimum inhibitory concentrations of ibuprofen, conventional antimicrobials, and EOCs were determined and then combined and tested against 14 (reference and clinical) skin pathogens. The cytotoxicity was analysed using the MTT assay, whilst the anti-inflammatory effects were evaluated using lipopolysaccharide activated RAW264.7 murine macrophages. Four pairwise (Ibuprofen and antibiotic) (ΣFIC 0.33-0.50) and three triple (Ibuprofen and antibiotic with EOC) (ΣFIC 0.44-0.47) synergistic antimicrobial interactions were identified. These combinations demonstrated cell viability of 77.59%-100%. No combination significantly reduced nitric oxide production.

Conclusion: The results from this study provide insight into the potential of a multidrug combination involving ibuprofen with conventional antimicrobials and EOCs against common skin pathogens.

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布洛芬作为传统抗菌剂和精油化合物的辅助剂，对抗皮肤病原体。

目的：抗菌药耐药性仍是一个日益令人担忧的问题，导致联合用药的使用越来越多。抗生素辅助剂是一种可增强抗生素疗效的新兴策略。布洛芬的多药理特性具有抗菌和调节宿主的潜力。本研究旨在探讨布洛芬、精油化合物和传统抗菌剂的新型多药组合对皮肤病原体的作用潜力：方法：测定布洛芬、传统抗菌剂和精油化合物的最小抑菌浓度，然后将其结合起来，对 14 种（参考和临床）皮肤病原体进行测试。细胞毒性采用 MTT 法进行分析，抗炎效果则采用脂多糖激活的 RAW264.7 小鼠巨噬细胞进行评估：结果：发现了四种成对抗菌作用（布洛芬和抗生素）（ΣFIC 0.33-0.50）和三种三重抗菌作用（布洛芬和抗生素与精油化合物）（ΣFIC 0.44-0.47）。这些组合的细胞存活率为 77.59%-100%。没有一种组合能明显减少一氧化氮的产生：本研究的结果让我们深入了解了布洛芬与传统抗菌剂和精油化合物的多药组合对常见皮肤病原体的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.