Investigation of atypical serological profiles for Epstein-Barr virus (EBV)

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS Journal of virological methods Pub Date : 2024-07-26 DOI:10.1016/j.jviromet.2024.115002
Vincent Portet Sulla , Amina Kadi , Lina Mouna , Honorine Fenaux , Hugo Cechura , Rana Rafek , Julia Lubrano Di Ciccone , Fairly Warnakulasuriya , Christelle Vauloup-Fellous
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Abstract

Background

Commercial immunoassays that detect IgG and IgM directed toward VCA and IgG EBNA are used in combination to assess EBV immune status. However, this strategy does not always confirm/exclude recent/past EBV infection or absence of immunity.

Objectives

The aim of our study was to perform complementary investigations on samples with atypical EBV serological profiles, in order to identify the clinical situation they correspond to.

Study design

EBV serology was performed using EBV VCA IgM/IgG and EBNA IgG LXL® DiaSorin assay. Complementary investigations included ELISA IgM VCA, immunoblots, CMV IgM/IgG and CMV IgG avidity, and EBV PCR.

Results

In our study, 12810 EBV serological results were analyzed, and 3580 atypical profiles were detected (28 %). Among these latter, isolated VCA IgG represented 42.9 %, the three positive markers accounted for 29.1 %, isolated EBNA IgG represented 18.5 %, isolated VCA IgM accounted for 6.4 % and positive VCA IgM & positive EBNA IgG represented 3.1 %. VCA IgG detected alone were specific in 100 % cases and EBNA IgG detected alone were specific in 91.7 % cases. VCA IgM detected alone were false positive or due to a cross reaction with CMV in 52.8 % cases. The pattern positive VCA IgM and positive EBNA IgG correspond to a false positive in VCA IgM, EBNA IgG or both in 83.4 % cases. Positive EBV VCA IgM/IgG and EBNA IgG were unreliable to detect active EBV infection in 66.7 % cases.

Discussion

Atypical EBV serological profiles may correspond to several clinical situations and complementary investigations allow to determine the immune status in more than 98.5 % cases.

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对 Epstein-Barr 病毒(EBV)非典型血清学特征的调查。
背景:检测针对 VCA 和 IgG EBNA 的 IgG 和 IgM 的商用免疫测定可用于联合评估 EBV 免疫状态。然而,这种策略并不总能确认/排除近期/既往的 EBV 感染或缺乏免疫力:我们的研究旨在对EBV血清学特征不典型的样本进行补充检查,以确定它们所对应的临床情况:研究设计:使用 EBV VCA IgM/IgG 和 EBNA IgG LXL® DiaSorin 检测法进行 EBV 血清学检测。辅助检查包括 ELISA IgM VCA、免疫印迹、CMV IgM/IgG 和 CMV IgG 阳性以及 EBV PCR:我们的研究分析了 12810 项 EBV 血清学结果,发现了 3580 项非典型特征(28%)。其中,分离的 VCA IgG 占 42.9%,三种阳性标记物占 29.1%,分离的 EBNA IgG 占 18.5%,分离的 VCA IgM 占 6.4%,阳性 VCA IgM 和阳性 EBNA IgG 占 3.1%。单独检测到的 VCA IgG 对 100% 的病例具有特异性,单独检测到的 EBNA IgG 对 91.7% 的病例具有特异性。单独检测出的 VCA IgM 呈假阳性或与 CMV 发生交叉反应的病例占 52.8%。在 83.4% 的病例中,VCA IgM 阳性和 EBNA IgG 阳性的模式对应于 VCA IgM、EBNA IgG 或两者的假阳性。在 66.7% 的病例中,EBV VCA IgM/IgG 阳性和 EBNA IgG 阳性对检测活动性 EBV 感染并不可靠:讨论:非典型 EBV 血清学特征可能与多种临床情况相对应,辅助检查可确定 98.5% 以上病例的免疫状态。
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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
209
审稿时长
41 days
期刊介绍: The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.
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