Comparison of different derivatisation for amino acids determination of foie gras by high performance liquid chromatography (HPLC).

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE British Poultry Science Pub Date : 2024-07-30 DOI:10.1080/00071668.2024.2375723

R Wei, Y Teng, R Ning, Z Luo, L Bai, C Han

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Abstract

1. In order to compare the difference between different derivatisations for amino acids determination of foie gras via, reversed phase high performance liquid chromatography (HPLC), O-phthalaldehyde and 9-fluorenyl-methyl chloroformate (OPA-FMOC group), phenylisothiocyanate (PITC group) and 6-Aminoquinolyl-N-hydrox-ysuccinimidyl Carbamate (AQC group) were applied to derivatisation reagent in this current experiment. The determination results of automatic amino acid analyser were applied, and 17 amino acids were detected by these three derivatisation methods.2. The running times of OPA-FMOC group, PITC group and AQC group were 18, 45 and 35 min, respectively. There was a large difference between the results of OPA-FMOC group and results from the automatic amino acid analyser, although the difference between the results from PITC and the automatic amino acid analyser was minimal.3. In conclusion, the running time of OPA-FMOC group was shorter than that of PITC group and AQC group; the accuracy of the former was better than the OPA-FMOC group and AQC group for the determination of amino acid of foie gras.

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通过高效液相色谱法（HPLC）测定鹅肝中氨基酸的不同衍生方法比较。

为了比较不同衍生法测定鹅肝氨基酸的差异，本实验采用反相高效液相色谱法（HPLC）、邻苯二甲醛和 9-芴基甲基氯甲酸酯衍生法（OPA-FMOC 衍生法）、苯基异硫氰酸酯衍生法（PITC 衍生法）和 6-氨基喹啉-N-羟基-琥珀酰亚胺基氨基甲酸酯衍生法（AQC 衍生法）对鹅肝进行衍生。2. OPA-FMOC 组、PITC 组和 AQC 组的衍生时间分别为 18、45 和 35 分钟。总之，在测定鹅肝氨基酸时，OPA-FMOC 组的运行时间比 PITC 组和 AQC 组短；前者的准确度优于 OPA-FMOC 组和 AQC 组。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .