Baicalin and probenecid protect against Glaesserella parasuis challenge in a piglet model.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES Veterinary Research Pub Date : 2024-07-29 DOI:10.1186/s13567-024-01352-4
Shulin Fu, Siyu Liu, Jingyang Li, Qiaoli Dong, Yunjian Fu, Ronghui Luo, Yamin Sun, Xinyue Tian, Wei Liu, Bingbing Zong, Chun Ye, Qirong Lu, Yinsheng Qiu, Ling Guo
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Abstract

Glaesserella parasuis (G. parasuis) induces vascular damage and systemic inflammation. However, the mechanism by which it causes vascular damage is currently unclear. Baicalin has important anti-inflammatory, antibacterial and immunomodulatory functions. In this study, we explored the ability of baicalin and probenecid to protect against G. parasuis challenge in a piglet model. Sixty piglets were randomly divided into a control group; an infection group; a probenecid group; and 25 mg/kg, 50 mg/kg and 100 mg/kg baicalin groups. The probenecid group and the 25 mg/kg, 50 mg/kg and 100 mg/kg baicalin groups were injected intramuscularly with 20 mg/kg body weight (BW) probenecid and 25 mg/kg BW, 50 mg/kg BW and 100 mg/kg BW baicalin, respectively. All piglets except those from the control group were injected intraperitoneally with 1 × 108 CFU of G. parasuis. The control group was injected intraperitoneally with TSB. The results showed baicalin and probenecid protected piglets against G. parasuis challenge, improved body weight and decreased temperature changes in piglets. Baicalin and probenecid attenuated IL-1β, IL-10, IL-18, TNF-α and IFN-γ mRNA levels in the blood for 48 h, inhibited the production of the nucleosides ATP, ADP, AMP and UMP from 24 to 72 h, reduced Panx-1/P2Y6/P2X7 expression, weakened NF-kB, AP-1, NLRP3/Caspase-1 and ROCK/MLCK/MLC signalling activation, and upregulated VE-cadherin expression in the blood vessels of piglets challenged with G. parasuis. Baicalin and probenecid alleviated pathological tissue damage in piglets induced by G. parasuis. Our results might provide a promising strategy to control and treat G. parasuis infection in the clinical setting.

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黄芩苷和丙磺舒可在仔猪模型中防止寄生璃泽氏菌挑战。
寄生蝙蝠(Glaesserella parasuis)会诱发血管损伤和全身炎症。然而,它造成血管损伤的机制目前还不清楚。黄芩苷具有重要的抗炎、抗菌和免疫调节功能。在这项研究中,我们探讨了黄芩苷和丙磺舒在仔猪模型中抵御寄生虫挑战的能力。60 头仔猪被随机分为对照组、感染组、丙磺舒组、25 毫克/千克、50 毫克/千克和 100 毫克/千克黄芩苷组。丙磺舒组和 25 毫克/千克、50 毫克/千克和 100 毫克/千克黄芩苷组分别肌肉注射 20 毫克/千克体重的丙磺舒和 25 毫克/千克体重、50 毫克/千克体重和 100 毫克/千克体重的黄芩苷。除对照组外,所有仔猪腹腔注射 1 × 108 CFU 的寄生虫。对照组腹腔注射 TSB。结果表明,黄芩苷和丙磺舒可保护仔猪免受寄生虫的侵袭,提高仔猪的体重,降低仔猪的体温变化。黄芩苷和丙磺舒可在 48 小时内降低血液中的 IL-1β、IL-10、IL-18、TNF-α 和 IFN-γ mRNA 水平,在 24 至 72 小时内抑制核苷酸 ATP、ADP、AMP 和 UMP 的产生、减少 Panx-1/P2Y6/P2X7 的表达,削弱 NF-kB、AP-1、NLRP3/Caspase-1 和 ROCK/MLCK/MLC 信号的激活,并上调受寄生虫挑战的仔猪血管中 VE-cadherin 的表达。寄生虫。黄芩苷和丙磺舒减轻了寄生虫对仔猪病理组织的损伤。我们的研究结果可能会为在临床环境中控制和治疗寄生虫感染提供一种有前景的策略。
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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