Electropenetrography with Alternating Current Reveals In Situ Changes of Aedes aegypti Probing Behaviors Associated with Dengue Virus Infection.

Abstract

Human infection with dengue virus (DENV) results in significant morbidity and mortality around the world. Current methods to investigate virus-associated changes in insect feeding behaviors are largely restricted to video analysis of feeding events outside of the host or intravital microscopy. Electropenetrography, a method originally developed for plant-feeding insects, offers a promising alternative by allowing high-resolution recording of voltage changes across the insect bite interface. We compared recordings from DENV-infected Aedes aegypti mosquitoes feeding on uninfected mice and uninfected A. aegypti feeding on DENV-infected mice to controls of uninfected A. aegypti feeding on uninfected mice. We found significant mosquito behavioral changes in both DENV-infected groups compared with controls including longer feeding times and longer preingestion probing events for A. aegypti feeding on DENV-infected mice and a higher number of sequential probing events in DENV-infected A. aegypti feeding on uninfected mice. By recording mosquito feeding and probing events beneath the surface of the skin, we have been able to both confirm and add new dimensions to previous findings regarding DENV-associated behavior changes in A. aegypti. This provides a foundation for increasingly in-depth studies focusing on the transmission of the DENV between vectors and hosts.