Evaluation of the Effect of Menopausal Status and BMI on Polymorphisms in Fas/Fas L and the Risk of Breast Cancer

IF 2.5 3区医学 Q2 ONCOLOGY Clinical breast cancer Pub Date : 2025-01-01 DOI:10.1016/j.clbc.2024.07.006

Zahra Tahmasebi Fard

{"title":"Evaluation of the Effect of Menopausal Status and BMI on Polymorphisms in Fas/Fas L and the Risk of Breast Cancer","authors":"Zahra Tahmasebi Fard","doi":"10.1016/j.clbc.2024.07.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>FAS and FAS ligand play an essential role in cell apoptosis. An identifying feature of malignant cells is the loss of FAS and increased FASL expression. A study analyzing the effects of menopausal status and body mass index (BMI) on functional polymorphisms of FAS-(1377G/A; rs2234767 & 670 A/G; rs1800682) and FASL (−844T/C; rs763110 & Ivs-2nt; rs5030772) in breast cancer evaluated these effects.</div></div><div><h3>Patients and Methods</h3><div>316 blood samples were collected from breast cancer patients and healthy controls in this case/control study. RFLP-PCR was used after DNA extraction to determine genotypes. Age, BMI, menopausal status, smoking, and family history were also analyzed with genotypes. It was analyzed using SPSS software, X2 statistical tests, logistic regression, and Pearson's correlation. The study evaluated the role of indices and polymorphisms in breast cancer risk.</div></div><div><h3>Results</h3><div>While BMI and family history were significantly different, age, menopause status, and smoking were not. Examining the average BMI between menopausal and nonmenopausal people in the 2 groups showed a statistically significant difference between menopausal people (<em>P</em> <0.0001). As a result of 1377AA, 670GG, 844TT, and IVS-2ntGG, the risk of breast cancer increased by 1.83 times, 2.35 times, and 2.38 times respectively. In addition, mutant alleles increased disease risk significantly. The risk of disease increased considerably for postmenopausal females with certain genotypes (except 1377GA and 844CT genotypes) and high BMI.</div></div><div><h3>Conclusion</h3><div>Having a high BMI during postmenopause increases your risk of breast cancer. In addition to menopause, BMI also influences disease progression. Different genotypes are needed to clarify this issue.</div></div>","PeriodicalId":10197,"journal":{"name":"Clinical breast cancer","volume":"25 1","pages":"Pages e56-e62"},"PeriodicalIF":2.5000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical breast cancer","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1526820924002039","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

FAS and FAS ligand play an essential role in cell apoptosis. An identifying feature of malignant cells is the loss of FAS and increased FASL expression. A study analyzing the effects of menopausal status and body mass index (BMI) on functional polymorphisms of FAS-(1377G/A; rs2234767 & 670 A/G; rs1800682) and FASL (−844T/C; rs763110 & Ivs-2nt; rs5030772) in breast cancer evaluated these effects.

Patients and Methods

316 blood samples were collected from breast cancer patients and healthy controls in this case/control study. RFLP-PCR was used after DNA extraction to determine genotypes. Age, BMI, menopausal status, smoking, and family history were also analyzed with genotypes. It was analyzed using SPSS software, X2 statistical tests, logistic regression, and Pearson's correlation. The study evaluated the role of indices and polymorphisms in breast cancer risk.

Results

While BMI and family history were significantly different, age, menopause status, and smoking were not. Examining the average BMI between menopausal and nonmenopausal people in the 2 groups showed a statistically significant difference between menopausal people (P <0.0001). As a result of 1377AA, 670GG, 844TT, and IVS-2ntGG, the risk of breast cancer increased by 1.83 times, 2.35 times, and 2.38 times respectively. In addition, mutant alleles increased disease risk significantly. The risk of disease increased considerably for postmenopausal females with certain genotypes (except 1377GA and 844CT genotypes) and high BMI.

Conclusion

Having a high BMI during postmenopause increases your risk of breast cancer. In addition to menopause, BMI also influences disease progression. Different genotypes are needed to clarify this issue.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

评估绝经状态和体重指数对 Fas/Fas L 多态性和乳腺癌风险的影响

FAS及其配体在细胞凋亡中起重要作用。恶性细胞的一个特征是FAS的缺失和FASL表达的增加。绝经状态和体重指数（BMI）对FAS-(1377G/A)功能多态性影响的研究；rs2234767,670 A / G;rs1800682)和FASL(−844T/C；rs763110,Ivs-2nt;Rs5030772)来评估这些影响。患者与方法本研究采集了316例乳腺癌患者和健康对照者的血液样本。提取DNA后，采用RFLP-PCR检测基因型。年龄、体重指数、绝经状态、吸烟和家族史也与基因型进行了分析。采用SPSS软件、X2统计检验、logistic回归、Pearson相关分析。该研究评估了指数和多态性在乳腺癌风险中的作用。结果BMI和家族史有显著性差异，年龄、绝经状态和吸烟无显著性差异。检查两组绝经期和非绝经期人群的平均BMI，绝经期人群之间的差异有统计学意义（P <0.0001）。1377AA、670GG、844TT和IVS-2ntGG分别增加了1.83倍、2.35倍和2.38倍的乳腺癌风险。此外，突变等位基因显著增加疾病风险。某些基因型（1377GA和844CT基因型除外）和高BMI的绝经后女性患病风险显著增加。结论：绝经后身体质量指数高会增加患乳腺癌的风险。除了更年期，BMI也会影响疾病的进展。需要不同的基因型来澄清这个问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Clinical breast cancer 医学-肿瘤学

CiteScore

5.40

自引率

3.20%

发文量

174

审稿时长

48 days

期刊介绍： Clinical Breast Cancer is a peer-reviewed bimonthly journal that publishes original articles describing various aspects of clinical and translational research of breast cancer. Clinical Breast Cancer is devoted to articles on detection, diagnosis, prevention, and treatment of breast cancer. The main emphasis is on recent scientific developments in all areas related to breast cancer. Specific areas of interest include clinical research reports from various therapeutic modalities, cancer genetics, drug sensitivity and resistance, novel imaging, tumor genomics, biomarkers, and chemoprevention strategies.