Host urinary biomarkers in HIV positive and HIV negative patients with tubercular uveitis and other uveitic diseases

IF 2.8 3区医学 Q3 IMMUNOLOGY Tuberculosis Pub Date : 2024-07-23 DOI:10.1016/j.tube.2024.102547

Dian P. van der Westhuizen , Candice I. Snyders , Martin Kidd , Gerhard Walzl , Novel N. Chegou , Derrick P. Smit

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Abstract

Purpose

To determine if host urinary biomarker profiles could distinguish between tubercular uveitis (TBU) and other uveitic diseases (OUD) in patients with and without HIV infection.

Methods

Concentrations of 29 different host biomarkers were measured in urine samples using the Luminex platform. Data were analyzed to describe differences between patients diagnosed with and without TBU and with and without HIV co-infection.

Results

One-hundred-and-eighteen urine samples were collected and 39% participants were diagnosed as TBU+. Mean age TBU+ was 39.3±13.6 years with 45.7% males. Anterior and panuveitis and unilateral involvement were most common. 32.6% were TBU+HIV+ (median CD4+=215) while 40.2% were OUD+HIV+ (median CD4+=234). Only sVEGF3 was decreased in TBU+ versus OUD+ (p=0.03), regardless of HIV status. Some biomarkers were significantly raised in HIV+ TBU+ compared to HIV- TBU+: sIL-6Rα, CD30, sRAGE , sTNFR I&-II, IP-10, MIP-1β, sEGFR and Ferritin. HIV+ OUD+ had increased sVEGFR3, CD30, sIL-6Rα, IP-10, sTNFR I&-II, Ferritin and Haptoglobin compared to HIV- OUD+. VEGF-A (p = 0.04) was decreased in HIV+ OUD+ versus HIV- OUD+.

Conclusion

Decreased urinary concentrations of VEGFR3 were observed in TBU+ compared to TBU-. HIV+ individuals demonstrated increased concentrations of multiple urinary analytes when compared to HIV- patients with uveitis.

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患有结核性葡萄膜炎和其他葡萄膜病的 HIV 阳性和 HIV 阴性患者的宿主尿液生物标志物

目的确定宿主尿液生物标志物图谱能否区分感染和未感染艾滋病病毒的患者中的结核性葡萄膜炎（TBU）和其他葡萄膜疾病（OUD）。结果收集了 118 份尿样，39% 的参与者被诊断为 TBU+。TBU+患者的平均年龄为 39.3±13.6 岁，男性占 45.7%。前葡萄膜炎和泛葡萄膜炎以及单侧受累最为常见。32.6%为TBU+HIV+（中位数CD4+=215），40.2%为OUD+HIV+（中位数CD4+=234）。只有 sVEGF3 在 TBU+ 与 OUD+ 之间有所下降（p=0.03），与 HIV 感染状况无关。与 HIV- TBU+ 相比，HIV+ TBU+ 的一些生物标志物明显升高：sIL-6Rα、CD30、sRAGE、sTNFR I&-II、IP-10、MIP-1β、sEGFR 和铁蛋白。与 HIV- OUD+ 相比，HIV+ OUD+ 的 sVEGFR3、CD30、sIL-6Rα、IP-10、sTNFR I&-II、铁蛋白和高铁血红蛋白均有所增加。VEGF-A（p = 0.04）在 HIV+ OUD+ 与 HIV- OUD+ 相比有所下降。与患有葡萄膜炎的 HIV- 患者相比，HIV+ 患者尿液中多种分析物的浓度升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.