Ignisphaera cupida sp. nov., a hyperthermophilic hydrolytic archaeon from a hot spring of Uzon (Kamchatka), and emended description of the genus Ignisphaera

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2024-07-26 DOI:10.1016/j.syapm.2024.126541
Olga A. Podosokorskaya, Alexander G. Elcheninov, Alexandra A. Klyukina, Alexander Y. Merkel
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Abstract

A novel strictly anaerobic hyperthermophilic archaeon, strain 4213-coT, was isolated from a terrestrial hot spring in the Uzon Caldera, Kamchatka (Russian Federation). Coccoid cells were present singly, in pairs, or aggregates, and occasionally were motile. The strain grew at 75–100 °C and within a pH range of 5.4–8.2 with the optimum at 92 °C and pH 6.4–6.7. Strain 4213-coT was a chemoorganoheterotroph, growing on proteinaceous substrates and mono-, di- and polysaccharides (starch, guar gum, xanthan gum). It did not require sodium chloride for growth. The complete genome of strain 4213-coT was 1.74 Mbp in size; its G+C content was 36.18 %. Genome analysis allowed to identify 25 genes encoding glycosidases involved in polysaccharide hydrolysis as well as genes of ADP-forming acetate-CoA ligase, lactate dehydrogenase and two [NiFe] hydrogenases responsible for acetate, lactate and hydrogen formation during fermentation. Moreover gene cluster encoding archaellum subunits was found. According to the phylogenomic analysis strain 4213-coT formed a species-level phylogenetic lineage within Ignisphaera genus. Our phylogenomic analysis also supports the delineation of the Ignisphaera genus into a separate family Ignisphaeraceae, as recently published. Here we propose a novel species Ignisphaera cupida, sp. nov. with type strain 4213-coT (=JCM 39446T=VKM B-3715T=UQM 41593T). Ecogenomic analysis showed that representatives of the Ignisphaera are thermophilic archaea, the majority of them were found in terrestrial hot springs and deep-sea hydrothermal vents. This study allowed a better understanding of physiology and ecology of Ignisphaeraceae – a rather understudied archaeal group.

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来自乌宗(堪察加半岛)温泉的嗜热水解古菌 Ignisphaera cupida sp.
从堪察加半岛(俄罗斯联邦)乌孙破火山口的陆地温泉中分离出了一种新的严格厌氧的嗜热古菌--4213-coT 菌株。球状细胞单个、成对或聚集存在,偶尔会运动。该菌株的生长温度为 75-100℃,pH 值范围为 5.4-8.2,最适生长温度为 92℃,pH 值为 6.4-6.7。菌株 4213-coT 是一种化学有机异养生物,可在蛋白质基质和单糖、双糖和多糖(淀粉、瓜尔豆胶、黄原胶)上生长。它的生长不需要氯化钠。菌株 4213-coT 的完整基因组大小为 1.74 Mbp,G+C 含量为 36.18%。通过基因组分析,确定了参与多糖水解的 25 个糖苷酶编码基因、ADP 形成醋酸-CoA 连接酶基因、乳酸脱氢酶基因以及在发酵过程中负责形成醋酸、乳酸和氢气的两个[NiFe]氢酶基因。此外,还发现了编码弓形体亚基的基因簇。根据系统发生组分析,菌株 4213-coT 在 Ignisphaera 属中形成了一个种级系统发生系。我们的系统发生组分析也支持最近发表的将 Ignisphaera 属划分为一个独立的 Ignisphaeraceae 科的观点。在此,我们提出了一个新物种 Ignisphaera cupida,新种,其模式菌株为 4213-coT(=JCM 39446T=VKM B-3715T=UQM 41593T)。生态基因组分析表明,Ignisphaera 的代表是嗜热古细菌,它们中的大多数在陆地温泉和深海热液喷口中被发现。这项研究有助于更好地了解嗜热古细菌的生理学和生态学。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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