Multimodal assessment of high-risk human papillomavirus in sinonasal squamous cell carcinoma

IF 2.9 4区医学 Q2 PATHOLOGY Pathology, research and practice Pub Date : 2024-07-23 DOI:10.1016/j.prp.2024.155486

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Abstract

High-risk human papillomavirus (hrHPV) is an emerging risk factor for sinonasal squamous cell carcinoma (SNSCC). The goal of this study was to assess the prevalence of hrHPV and subtype distribution in SNSCC and correlation with patient and clinical characteristics. This retrospective cohort study included 43 cases diagnosed with incident primary SNSCC at the University of Cincinnati Medical Center from 2010 to 2015. The prevalence of hrHPV was interrogated using a multi-assay approach that included p16 immunohistochemistry (IHC), RNA in-situ hybridization (ISH), and hrHPV DNA sequencing. The association of hrHPV with 5-year overall survival (OS) and 2-year disease-free survival (DFS) was assessed. Fourteen cases (32.6 %) were classified as hrHPV positive, based on the a priori definition of having either a positive RNAScope™ ISH test or hrHPV DNA and p16-positive IHC; 9 cases (20.9 %) were positive for all three tests. All cases that arose from an inverted sinonasal papilloma (ex-ISP) were negative for hrHPV. HPV16 was the most common subtype among hrHPV positive cases (58.8 %), followed by HPV18 (17.6 %). No significant association was observed between hrHPV and OS or DFS after adjusting for potential confounding. hrHPV is prevalent in a sizable fraction of SNSCC. Additional studies are needed to better elucidate the relationship with patient survival outcomes and determine the optimal testing modality for prognostication.

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鼻窦鳞状细胞癌中高危人类乳头瘤病毒的多模式评估

高危人乳头瘤病毒（hrHPV）是鼻窦鼻腔鳞状细胞癌（SNSCC）的新风险因素。本研究旨在评估hrHPV在鼻窦鳞状细胞癌中的流行率和亚型分布，以及与患者和临床特征的相关性。这项回顾性队列研究纳入了 2010 年至 2015 年期间在辛辛那提大学医学中心确诊的 43 例原发性 SNSCC 病例。研究人员采用多种检测方法检测了hrHPV的流行情况，包括p16免疫组化（IHC）、RNA原位杂交（ISH）和hrHPV DNA测序。评估了hrHPV与5年总生存期（OS）和2年无病生存期（DFS）的关系。根据 RNAScope™ ISH 检测阳性或 hrHPV DNA 和 p16 IHC 阳性的先验定义，14 例病例（32.6%）被归类为 hrHPV 阳性；9 例病例（20.9%）三项检测均为阳性。所有来自倒置鼻窦乳头状瘤（ex-ISP）的病例均为 hrHPV 阴性。在 hrHPV 阳性病例中，HPV16 是最常见的亚型（58.8%），其次是 HPV18（17.6%）。在调整了潜在的混杂因素后，未观察到 hrHPV 与 OS 或 DFS 之间有明显的关联。为了更好地阐明hrHPV与患者生存结果的关系并确定预后的最佳检测方式，还需要进行更多的研究。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.