RNA modifications identification based on chemical reactions

IF 3.3 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioorganic & Medicinal Chemistry Pub Date : 2024-07-28 DOI:10.1016/j.bmc.2024.117861
Zhengjia Zhao , Weikai Yan , Xiaocheng Weng
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Abstract

RNA modification identification is an emerging field in epigenetics due to its indispensable regulatory role in the cell life cycle. With advancements in identification methods, an increasing number of RNA modifications has been discovered, thereby driving the development of more efficient and accurate techniques for localizing modified RNAs and elucidating their functions. High-throughput sequencing approaches for modified RNA detection can be categorized into antibody-based, enzymatic-based, and chemical-labeling-based methods. Given the intrinsic chemical reactions involved in all biochemical processes, we provide a comprehensive review of recent advancements in artificial chemical labeling and transformations of ten distinct RNA modifications and their applications in sequencing. Our aim is to contribute to a deeper understanding of the mechanisms underlying these modifications. We focus on the chemical reactions associated with RNA modifications and briefly compare the advantages and disadvantages of detection methods based on these reactions. Additionally, we introduce several approaches that identify multiple modifications through chemical labeling. As the field of RNA modification research continues to expand, we anticipate that the techniques and insights presented in this review will serve as a valuable resource for future studies aimed at further elucidating the functional roles of RNA modifications in biological processes.

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基于化学反应的 RNA 修饰鉴定。
由于 RNA 在细胞生命周期中发挥着不可或缺的调控作用,因此 RNA 修饰鉴定是表观遗传学的一个新兴领域。随着鉴定方法的进步,越来越多的 RNA 修饰被发现,从而推动了更高效、更准确的定位修饰 RNA 并阐明其功能的技术的发展。用于检测修饰 RNA 的高通量测序方法可分为基于抗体的方法、基于酶的方法和基于化学标记的方法。鉴于所有生化过程都涉及内在的化学反应,我们全面综述了十种不同 RNA 修饰的人工化学标记和转化的最新进展及其在测序中的应用。我们的目的是加深对这些修饰机理的理解。我们重点关注与 RNA 修饰相关的化学反应,并简要比较了基于这些反应的检测方法的优缺点。此外,我们还介绍了几种通过化学标记鉴定多种修饰的方法。随着 RNA 修饰研究领域的不断扩展,我们预计本综述中介绍的技术和见解将成为未来研究的宝贵资源,旨在进一步阐明 RNA 修饰在生物过程中的功能作用。
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来源期刊
Bioorganic & Medicinal Chemistry
Bioorganic & Medicinal Chemistry 医学-生化与分子生物学
CiteScore
6.80
自引率
2.90%
发文量
413
审稿时长
17 days
期刊介绍: Bioorganic & Medicinal Chemistry provides an international forum for the publication of full original research papers and critical reviews on molecular interactions in key biological targets such as receptors, channels, enzymes, nucleotides, lipids and saccharides. The aim of the journal is to promote a better understanding at the molecular level of life processes, and living organisms, as well as the interaction of these with chemical agents. A special feature will be that colour illustrations will be reproduced at no charge to the author, provided that the Editor agrees that colour is essential to the information content of the illustration in question.
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