Crosstalk between cytokines, inflammation and pulmonary arterial hypertension in heart transplant patients

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2024-07-29 DOI:10.1016/j.cyto.2024.156709
Marcus Vinícius de Paula da Silva , Ildernandes Vieira Alves , Antonielle Rodrigues Pereira Alves , Virginia Soares Lemos , Gabriel Assis Lopes do Carmo , Fábio Morato de Castilho , Cláudio Léo Gelape
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Abstract

Background: Heart transplant (HT) is a therapeutic option for patients with advanced heart failure (HF) refractory to optimized treatment. Patients with advanced HF often develop pulmonary arterial hypertension (PAH). PAH is defined as a condition in which the mean pulmonary artery pressure is greater than 20 mmHg. Inflammation is an important aspect of PAH development. In this context, the objective of this work was to evaluate the relationship between the inflammatory process and the development of HAP in patients undergoing HT. Methods: The levels of interleukins IL-6, IL-1β and TNF-α were obtained by ELISA and associated with CD68+ and CD66b neutrophil counts using the immunofluorescence technique in fragments of the pulmonary arteries of donors and patients with or without chagasic cardiomyopathy subjected to HT. Results: The results showed a positive, statistically significant correlation (p < 0.05) between right atrium pressure levels and IL-6. Furthermore, negative, moderate, and statistically significant correlations (p < 0.05) were observed between the variables cardiac index and TNF-α, and between the levels of transpulmonary pressure grandient and TNF-α. The study also revealed the presence of a statistically significant difference (p < 0.05) between patients who died within 30 days and the highest number of CD68 cells per square micrometer in the vessel of the donor and recipient patient. Conclusion: Suggesting the presence of a pro-inflammatory profile in HT patients, independent of measured pulmonary artery pressure levels.

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心脏移植患者的细胞因子、炎症和肺动脉高压之间的相互关系。
背景:心脏移植(HT)是对优化治疗无效的晚期心力衰竭(HF)患者的一种治疗选择。晚期心力衰竭患者通常会出现肺动脉高压(PAH)。PAH的定义是平均肺动脉压力超过20毫米汞柱。炎症是 PAH 发展的一个重要方面。在此背景下,本研究的目的是评估接受高流量治疗的患者的炎症过程与 HAP 发生之间的关系:方法:采用 ELISA 方法检测白细胞介素 IL-6、IL-1β 和 TNF-α的水平,并采用免疫荧光技术检测供体肺动脉片段中 CD68+ 和 CD66b 中性粒细胞的数量,以及接受 HT 治疗的恰加斯性心肌病或非恰加斯性心肌病患者的肺动脉片段中 CD68+ 和 CD66b 中性粒细胞的数量:结果表明,两者之间存在统计学意义上的正相关(P结果:结果显示,两者之间存在统计学意义上的正相关(p)。
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来源期刊
Cytokine
Cytokine 医学-免疫学
CiteScore
7.60
自引率
2.60%
发文量
262
审稿时长
48 days
期刊介绍: The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.
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