Anticancer Activity of Iso-Mukaadial Acetate on Pancreatic and Colon Cancer Cells.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2024-01-01 DOI:10.61186/rbmb.12.4.586
Portia Raphela-Choma, Lesetja Motadi, Mthokosizi Simelane, Mpho Choene
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Abstract

Background: Pancreatic cancer and colon cancer pose significant challenges in treatment, with poor prognoses. Natural products have long been explored for their potential as anticancer agents. Iso-mukaadial acetate has shown promise in inducing apoptosis in breast and ovarian cancer cells. The objective of this study was to investigate the effect of Iso-mukaadial acetate on pancreatic (MIA-PACA2) and colon (HT29) cancer cell lines.

Methods: Pancreatic (MIA-PACA2) cancer cells, colon (HT29) cancer cells, normal embryonic kidney cells (HEK 293), and normal lung cells (MRC5) were cultured and treated with Iso-mukaadial acetate (IMA) for 24 hours. The viability assays were conducted using Alamarblue reagent and a real-time cell viability monitoring system, xCELLigence. The IC50 values were determined, followed by assessments of ATP production, caspase 3/7 activation, mitochondrial function, morphological changes using a light microscope, and gene expression changes via RT-PCR.

Results: This study indicates that Iso-mukaadial acetate exhibited concentration-dependent cytotoxic effects, slowing cellular proliferation in both cancer cell lines. Activation of the mitochondrial apoptotic pathway and caspase 3/7 suggests induction of apoptosis. Reduced ATP production and altered gene expression further support its anticancer properties. Morphological changes after treatment with Iso-mukaadial acetate showed apoptotic characteristics which may suggest that apoptosis was induced.

Conclusions: According to the results obtained, Iso-mukaadial acetate shows potential as an anticancer agent, evidenced by its effects on cellular viability, mitochondrial function, ATP production, caspase activation, and gene expression in pancreatic and colon cancer cells. These findings highlight its promise for further investigation and potential in the development of therapeutic agents.

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醋酸异木瓜醛对胰腺癌和结肠癌细胞的抗癌活性
背景:胰腺癌和结肠癌预后不良,给治疗带来巨大挑战。长期以来,人们一直在探索天然产品作为抗癌剂的潜力。异木瓜醇醋酸酯有望诱导乳腺癌和卵巢癌细胞凋亡。本研究旨在探讨异木瓜醇醋酸酯对胰腺癌(MIA-PACA2)和结肠癌(HT29)细胞系的影响:方法:培养胰腺癌细胞(MIA-PACA2)、结肠癌细胞(HT29)、正常胚胎肾细胞(HEK 293)和正常肺细胞(MRC5),并用醋酸异木瓜酯(IMA)处理 24 小时。细胞活力检测采用 Alamarblue 试剂和实时细胞活力监测系统 xCELLigence 进行。确定了 IC50 值,然后评估了 ATP 生成、caspase 3/7 激活、线粒体功能、光镜下的形态变化以及 RT-PCR 下的基因表达变化:研究表明,醋酸异木瓜酯具有浓度依赖性细胞毒性作用,可减缓两种癌细胞株的细胞增殖。线粒体凋亡途径和 caspase 3/7 的激活表明诱导了细胞凋亡。ATP 生成的减少和基因表达的改变进一步支持了它的抗癌特性。醋酸异木瓜酯处理后的形态变化显示出凋亡特征,这可能表明诱导了细胞凋亡:根据研究结果,醋酸异木瓜酯对胰腺癌和结肠癌细胞的细胞活力、线粒体功能、ATP 生成、Caspase 激活和基因表达都有影响,这证明它具有抗癌潜力。这些发现凸显了它在进一步研究和开发治疗药物方面的前景和潜力。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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