Exogenous Ceramide Treatment Induce Death and Cytotoxicity in Glioma Cells.

Turkish neurosurgery Pub Date : 2024-01-01 DOI:10.5137/1019-5149.JTN.44624-23.3

Murat Baloglu, Canan Vejselova Sezer, Gizem Karadağ, Erdal Yayla, Hatice Mehtap Kutlu

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Abstract

Aim: To evaluate the cytotoxic and proapoptotic effects of C6 ceramide on the C6 rat glioma cell line.

Material and methods: The C6 rat glioma cell line was evaluated. Using a confocal microscope and the appropriate software, the cytotoxic effects of C6 ceramide were identified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) colorimetric experiments. Transmission electron microscopy (TEM) was utilized to examine the ultrastructural changes following treatment with IC50 concentrations of C6 ceramide.

Results: Condensation and fragmentation of nuclei and DNA laddering was observed, indicating apoptotic cell death. C6 ceramide induced apoptosis and effectively caused cytotoxicity in the C6 glioblastoma cells. MTT assay demonstrated > 90% cell death after short-term application of C6 ceramide, confirming its apoptosis-triggering effect. Apoptosis was also confirmed via confocal microscopy and TEM.

Conclusion: Glioblastoma cells undergo apoptosis when exposed to C6 ceramide, which makes it a potential chemotherapeutic agent for the treatment of this aggressive brain cancer.

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外源性神经酰胺处理诱导胶质瘤细胞死亡和细胞毒性

目的：多形性胶质母细胞瘤是最常见的中枢神经系统恶性肿瘤。尽管采用了当前的治疗策略，但复发率和死亡率仍然很高。据报道，神经酰胺是细胞凋亡和生长抑制等抗增殖反应的主要介质。我们旨在评估C6神经酰胺对C6大鼠胶质瘤细胞系的细胞毒性和促凋亡作用：材料和方法：对 C6 大鼠胶质瘤细胞系进行了评估。使用共聚焦显微镜和相应的软件，通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基-2H 溴化四氮唑（MTT）比色实验确定了 C6 神经酰胺的细胞毒性作用。利用透射电子显微镜（TEM）检查了 IC50 浓度的 C6 神经酰胺处理后的超微结构变化：观察到细胞核凝结、破碎和 DNA 梯状化，表明细胞凋亡。C6 神经酰胺可诱导 C6 胶质母细胞瘤细胞凋亡，并有效地产生细胞毒性。MTT 检测显示，短期使用 C6 神经酰胺后，90% 的细胞死亡，证实了它的凋亡触发效应。共聚焦显微镜和 TEM 也证实了细胞凋亡：结论：胶质母细胞瘤细胞在接触 C6 神经酰胺后会发生凋亡，这使其成为治疗这种侵袭性脑癌的潜在化疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Turkish neurosurgery

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