Microalgal lipid production: A comparative analysis of Nannochloropsis and Microchloropsis strains

IF 2.8 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Phycology Pub Date : 2024-07-30 DOI:10.1007/s10811-024-03318-7

Omnia H. Abdelkarim, Rene H. Wijffels, Maria J. Barbosa

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Abstract

The oleaginous genera Nannochloropsis and Microchloropsis are recognized for their lipid accumulation capacity. Microalgal lipid accumulation is triggered by nitrogen starvation, negatively affecting photosynthesis and growth. Moreover, light and temperature play pivotal roles in microalgal physiology, lipid accumulation and composition. This study focuses on comparing the responses of eight microalgal strains from Nannochloropsis (N. oceanica Necton, N. oceanica IMET1, Nannochloropsis. sp. CCAP211/78, N. oculata, and N. limnetica) and Microchloropsis (M. gaditana CCFM01, M. gaditana CCMP526, and M. salina) to light, temperature, and nitrogen availability. Biomass, lipid content and productivities were monitored under different light intensities (150 (LL) and 600 μmol photons m⁻² s⁻¹ (HL)) and temperatures (15, 25, 30℃) under nitrogen (N-) starvation and replete conditions. Under N-starvation and HL, N. sp. exhibited the highest lipid content (59%) and productivity (0.069 g L^-1 day^-1), while N. oculata had the lowest lipid content (37.5%) and productivity (0.037 g L^-1 day^-1) among the eight strains. Notably, M. gaditana CCFM01 achieved the highest EPA content (4.7%), contrasting with N. oceanica IMET1 lowest EPA content (2.9%) under 150 μmol photons m⁻² s⁻¹ and N-repletion. The response to temperature fluctuations under LL was strain-dependent. Microchloropsis salina and M. gaditana CCFM01 demonstrated the highest and lowest lipid productivities (0.069 g L^-1 day^-1 and 0.022 g L^-1 day^-1, respectively) at 15℃ under N-starvation. Moreover, significant EPA accumulation across various strains was observed in N. oculata (5.7%) under N-repletion at 15°C, surpassing M. gaditana CCFM01 by 40%. Ultimately, the physiological responses to cultivation conditions vary markedly among microalgal strains, even within the same genus or species. This knowledge is essential for selecting suitable strains for the efficient microalgal lipid production industry.

Graphical Abstract

Optimi zing cultivation conditions for the maximal lipid production in Nannochloropsis andMicrochloropsis

Abstract Image

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微藻脂质生产：Nannochloropsis 和 Microchloropsis 菌株的比较分析

油藻属 Nannochloropsis 和 Microchloropsis 的脂质积累能力是公认的。氮饥饿会引发微藻脂质积累，从而对光合作用和生长产生负面影响。此外，光照和温度对微藻的生理、脂质积累和组成起着关键作用。本研究重点比较了 Nannochloropsis（N. oceanica Necton、N. oceanica IMET1、Nannochloropsis.在不同光照强度（150 μmol photons m-2 s-1 (LL)和 600 μmol photons m-2 s-1 (HL)）和温度（15、25、30℃）下，在氮饥饿和氮充足条件下，对生物量、脂质含量和生产率进行了监测。在氮饥饿和高光照条件下，N. sp. 的脂质含量（59%）和生产率（0.069 g L-1 day-1）最高，而 N. oculata 的脂质含量（37.5%）和生产率（0.037 g L-1 day-1）最低。值得注意的是，M. gaditana CCFM01 的 EPA 含量最高（4.7%），而 N. oceanica IMET1 在 150 μmol photons m-2 s-1 和氮补给条件下的 EPA 含量最低（2.9%）。在 LL 条件下，对温度波动的反应与菌株有关。在 15℃ N-饥饿条件下，Microchloropsis salina 和 M. gaditana CCFM01 的脂质生产率最高和最低（分别为 0.069 g L-1 day-1 和 0.022 g L-1 day-1）。此外，在 15℃缺氮条件下，观察到 N. oculata（5.7%）的 EPA 在不同菌株中都有显著积累，比 M. gaditana CCFM01 高出 40%。归根结底，不同微藻菌株对栽培条件的生理反应存在明显差异，即使是同一属或同一物种。这些知识对于为高效微藻脂质生产工业选择合适的菌株至关重要。

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来源期刊

Journal of Applied Phycology 生物-海洋与淡水生物学

CiteScore

6.80

自引率

9.10%

发文量

212

审稿时长

2.8 months

期刊介绍： The Journal of Applied Phycology publishes work on the rapidly expanding subject of the commercial use of algae. The journal accepts submissions on fundamental research, development of techniques and practical applications in such areas as algal and cyanobacterial biotechnology and genetic engineering, tissues culture, culture collections, commercially useful micro-algae and their products, mariculture, algalization and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds, antibiotics and other biologically active compounds. Each issue of the Journal of Applied Phycology also includes a short section for brief notes and general information on new products, patents and company news.