Biosynthesized Graphene Oxide Nanoparticles: In-Vitro Comparative Study for Biomedical Applications

IF 3.3 4区 物理与天体物理 Q2 CHEMISTRY, PHYSICAL Plasmonics Pub Date : 2024-07-30 DOI:10.1007/s11468-024-02433-y
Inas S. Mohammed, Duaa Hammoud, Sajidah H. Alkhazraji, Kareem H. Jawad, Buthenia A. Hasoon, Ali Abdullah Issa, Majid S. Jabir
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Abstract

Graphene-based materials have been the subject of extensive research due to its exceptional ability to kill a diverse array of microorganisms. The benefits of graphene-based materials include ease of fabrication, renewable resources, special catalytic properties, and remarkable physical properties including tensile strength and a large specific surface area. Our study utilizes an environmental method (laser production) to produce GONPs. GONPs are tested as potential; this study assesses the molecular docking simulation, anti-microbial, against clinically pathogenic strains of Klebsiella pneumoniae and Bacillus cereus. Antioxidant by DPPH assay and anti-cancer properties of graphene nanoparticles (GONPs) with Doxorubicin on lung cancer (A549 cell line). TEM images demonstrated types of produced GO-NP spherical nanoparticles with a size ranging at approximately 15–40 nm. Atomic force microscopy (AFM) was used to examine the morphological and topological characteristics of the NPs. The structural and crystal characteristics were examined by X-ray diffraction (XRD). Among the anti-bacterial-evaluated GONPs, concentrations of 100, 50, and 25 µg/ml exhibited the most substantial growth inhibition zone against Klebsiella pneumoniae and Bacillus cereus. The molecular docking simulation of GONP-OH modified gave more effective results against Bacillus cereus bacterial organism (ID: 5V8D) and (ID: 5GT6). Conversely, the highest anti-biofilm activity was observed against Bacillus cereus than Klebsiella pneumoniae, notably with 100 µg/ml GONPs. On the toxicity examination of cancer cells, the impact of nanoparticles was investigated. The produced nanoparticles had a higher cytotoxicity rate. The cytotoxicity of GONP alone, Doxorubicin alone, and/or combination therapy (GONP + Doxorubicin) found to be in 25 µg/ml concentration and time dependent manner also increased as combination therapy. The analysis for cell cytotoxicity revealed a noteworthy decrease in the number of cancer cells after GONP + Doxorubicin were treated for 72 h. The average cell cytotoxicity of GONP +Doxorubicin were 54, 61.31, and 76.41% for 24, 48, and 72 h, respectively. Both GONPs exhibited higher cell toxicity and cell death contract control. Additional GONPs showed strong antioxidant properties by DPPH assay. The present research demonstrates the advantageous effectiveness of a simpler production procedure, like laser production, for producing high-purity nanoparticles with low hazard that may be utilized as future possible cancer therapies.

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生物合成的氧化石墨烯纳米颗粒:用于生物医学应用的体外比较研究
石墨烯基材料具有杀死各种微生物的特殊能力,因此一直是广泛研究的主题。石墨烯基材料的优点包括易于制造、资源可再生、具有特殊的催化特性,以及包括抗拉强度和大比表面积在内的显著物理特性。我们的研究利用一种环保方法(激光生产)来生产 GONPs。本研究评估了分子对接模拟、抗微生物、抗临床致病性肺炎克雷伯氏菌和蜡样芽孢杆菌菌株的潜力。石墨烯纳米颗粒(GONPs)的 DPPH 抗氧化性和多柔比星对肺癌(A549 细胞系)的抗癌特性。TEM 图像显示了所制备的 GO-NP 球形纳米粒子的类型,其大小约为 15-40 纳米。原子力显微镜(AFM)用于检查 NPs 的形态和拓扑特征。X 射线衍射(XRD)检查了结构和晶体特征。在经过抗菌评估的 GONPs 中,浓度为 100、50 和 25 µg/ml 的 GONPs 对肺炎克雷伯氏菌和蜡样芽孢杆菌的生长抑制面积最大。分子对接模拟结果表明,改性 GONP-OH 对蜡样芽孢杆菌(ID:5V8D)和(ID:5GT6)的抑制效果更好。相反,与肺炎克雷伯菌相比,100 µg/ml GONPs 对蜡样芽孢杆菌的抗生物膜活性最高。在癌细胞毒性检测方面,研究了纳米颗粒的影响。生产的纳米粒子具有更高的细胞毒性。研究发现,单用 GONP、单用多柔比星和/或联合疗法(GONP + 多柔比星)的细胞毒性浓度为 25 微克/毫升,而且随着时间的推移,联合疗法的细胞毒性也会增加。细胞毒性分析表明,在 GONP + 多柔比星治疗 72 小时后,癌细胞数量显著减少。GONP + 多柔比星在 24、48 和 72 小时内的平均细胞毒性分别为 54%、61.31% 和 76.41%。两种 GONP 的细胞毒性和细胞死亡率均高于对照组。通过 DPPH 检测,其他 GONPs 还显示出很强的抗氧化性。本研究表明,激光生产等更简单的生产程序在生产高纯度、低危害的纳米粒子方面具有优势,可用作未来可能的癌症疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plasmonics
Plasmonics 工程技术-材料科学:综合
CiteScore
5.90
自引率
6.70%
发文量
164
审稿时长
2.1 months
期刊介绍: Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons. Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.
期刊最新文献
Comparative Analysis of Two Different MIM Configurations of a Plasmonic Nanoantenna On the Transmission Line Analogy for Modeling Plasmonic Nanowire Circuits Terahertz-Multiplexed Metallic Metasurfaces for Enhanced Trace Sample Absorption Plasmonic Characteristics of LiF Filled Slab Waveguide in Isotropic Plasma Environment Synthesis, Characterization, and Modeling of Reduced Graphene Oxide Supported Adsorbent for Sorption of Pb(II) and Cr(VI) Ions from Binary Mixture
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