Rapid and simultaneous detection of Salmonella Sp. and Staphylococcus aureus in seafood by Multiplex PCR (mPCR)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-07-30 DOI:10.2174/0115734110306807240724064141

Anusuya M, Praveena A

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Abstract

aims: The present study is to investigate the prevalence of Salmonella sp. and Staphylococcus in seafood sample using multiplex polymerase chain reaction (mPCR). background: The rapid detection of food borne pathogen is increasing to ensure safety to consumers as major food borne illness is caused by pathogenic bacteria. Salmonellosis caused by Salmonella sp. is one of the primary concerns in many countries. Staphylococcus aureus is capable of generating toxins which can produce food poisoning in human body. objective: The thermostable nuclease (nuc) gene of Staphylococcus aureus and enterotoxin (stn) gene of Salmonella were used as target genes for mPCR detection. method: Totally, 10 seafood which includes fishes, crab and prawns which are generally available in Indian fish markets were selected for the present study. Samples that carried both the strains Salmonella and Staphylococcus were selected for mPCR by targeting the stn and nuc gene. result: Among 10 seafood samples collected, 7 of them carried Salmonella strain and 5 of them carried Staphylococcus strains. The results showed that 75% of the salmonella strains carried stn gene and 75% of the Staphylococcus strains carried nuc gene. conclusion: This study suggests that mPCR can be used for simultaneous detection by targeting the stn gene and nuc gene of salmonella and Staphylococcus food borne pathogens in seafood. other: Not applicable

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利用多重 PCR (mPCR) 同时快速检测海产品中的沙门氏菌和金黄色葡萄球菌

目的：本研究采用多重聚合酶链反应（mPCR）技术调查海产品样本中沙门氏菌和葡萄球菌的流行率：本研究使用多重聚合酶链反应（mPCR）检测海产品样本中沙门氏菌和葡萄球菌的流行率：由于主要的食源性疾病是由致病菌引起的，为了确保消费者的安全，对食源性病原体的快速检测越来越多。由沙门氏菌引起的沙门氏菌病是许多国家主要关注的问题之一。金黄色葡萄球菌能产生毒素，可导致人体食物中毒：将金黄色葡萄球菌的恒温核酸酶（nuc）基因和沙门氏菌的肠毒素（stn）基因作为 mPCR 检测的目标基因：本研究共选择了 10 种海产品，包括鱼类、蟹类和虾类，这些海产品在印度的鱼类市场上都能买到。针对 stn 和 nuc 基因，选择同时携带沙门氏菌和葡萄球菌的样本进行 mPCR 检测：在收集到的 10 个海产品样本中，有 7 个携带沙门氏菌菌株，5 个携带葡萄球菌菌株。结果显示，75% 的沙门氏菌株携带 stn 基因，75% 的葡萄球菌株携带 nuc 基因：本研究表明，mPCR 可用于同时检测海产品中沙门氏菌和葡萄球菌食源性致病菌的 stn 基因和 nuc 基因：不适用

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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