Ballota acetabulosa (L). Bentham: A First-Time GC-MS Analysis and Antioxidant Potential of its Essential Oil.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Combinatorial chemistry & high throughput screening Pub Date : 2024-07-31 DOI:10.2174/0113862073319327240614113859

Ayse Nur Yazgan-Ekici, Filiz Bakar-Ates, Sinem Aslan Erdem, Betul Sever-Yilmaz

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Abstract

Background: Ballota acetabulosa native to the Mediterranean region, belonging to the Lamiaceae family, holds significance in folk medicine. Externally, it is applied for treating cuts and burns, while internally, it is utilized to alleviate inflammation, suppress cough, and address gastrointestinal issues.

Methods: This study aimed to investigate the chemical composition of the essential oil of Ballota acetabulosa and to evaluate the antioxidant capacity of the essential oil, as well as the aqueous and ethanolic extracts of the plant. Essential oil analysis was performed using Gas Chromatography- Mass Spectrometry (GC-MS), while 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and inhibition of lipid peroxidation assays were applied to the essential oil, water, and ethanol extracts of the plant.

Results: Spathulenol was found to be the predominant constituent of the essential oil, comprising 25.03% of the oil. Compared to the control group (Propyl gallate for DPPH, IC50 0.109; BHT for inhibition of lipid peroxidation, IC50 0.133), the essential oil was found to have insignificant antioxidant activity (IC50 value 10.395 mg/mL for DPPH, 1.051 mg/mL for inhibition of lipid peroxidation). Moreover, ethanolic extract (IC50 value 1.583 mg/mL for DPPH, 0.029 mg/mL for inhibition of lipid peroxidation) exerted more antioxidant activity than aqueous extract (IC50 value 1.9017 mg/mL for DPPH, 0.161 mg/mL for inhibition of lipid peroxidation).

Conclusion: Hitherto, this is the earliest report on the composition and activity of the essential oil Ballota acetabulosa. However, further investigation of different antioxidant capacity assays is suggested to highlight potential variations in mechanisms of action and subsequent results. Everything considered, this study advances the comprehension of the chemical composition and possible therapeutic uses of Ballota acetabulosa, highlighting the need for more research into its uses.

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Ballota acetabulosa (L).本瑟姆：首次 GC-MS 分析及其精油的抗氧化潜力。

背景：百洛塔（Ballota acetabulosa）原产于地中海地区，属于唇形科植物，在民间医药中具有重要地位。它外用可治疗割伤和烧伤，内服可缓解炎症、镇咳和解决肠胃问题：本研究旨在调查 Ballota acetabulosa 精油的化学成分，评估精油以及水提取物和乙醇提取物的抗氧化能力。精油分析采用气相色谱-质谱法（GC-MS），1,1-二苯基-2-苦基肼（DPPH）自由基清除活性和脂质过氧化抑制试验则应用于植物精油、水和乙醇提取物：结果：发现刺五加烯醇是精油的主要成分，占精油的 25.03%。与对照组（没食子酸丙酯对 DPPH 的 IC50 值为 0.109；BHT 对抑制脂质过氧化的 IC50 值为 0.133）相比，发现精油的抗氧化活性并不显著（对 DPPH 的 IC50 值为 10.395 毫克/毫升，对抑制脂质过氧化的 IC50 值为 1.051 毫克/毫升）。此外，乙醇提取物（DPPH 的 IC50 值为 1.583 mg/mL，抑制脂质过氧化的 IC50 值为 0.029 mg/mL）比水提取物（DPPH 的 IC50 值为 1.9017 mg/mL，抑制脂质过氧化的 IC50 值为 0.161 mg/mL）具有更强的抗氧化活性：到目前为止，这是关于百龄草精油成分和活性的最早报告。不过，建议进一步研究不同的抗氧化能力测定方法，以突出作用机制和后续结果的潜在差异。综上所述，本研究推动了人们对百合科植物乙酰百合的化学成分和可能的治疗用途的理解，突出了对其用途进行更多研究的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.