Statistical approach for newly isolated and identified microbial lipases production

Abstract

United Nations sustainable development goals (SDGs) are mended for the betterment of all living species on this earth. Climate change planning consistently revolves around sustainable products derived from natural raw materials. Our recent research has specifically targeted the optimization of biocatalyst production and microorganism growth through solid-state fermentation. In this research work, we utilized Aspergillus niger [S1], isolated from a mixed vegetable pickle. Initially, batch tests were conducted, altering the concentrations of five ingredients—sucrose, molasses, yeast extract, sunflower oil, and Tween-80 to achieve maximum extracellular biocatalyst (Lipases) production. Maximum extracellular biocatalyst activity was achieved in the presence of sucrose, molasses, yeast extract, sunflower oil and tween-80 at the rate of 6.39 ± 1.73^a U/mL, 2.2 ± 1.09^a U/mL, 2.22 ± 0.48^a U/mL, 3.06 ± 1.27^a U/mL and 2 ± 0.87^a U/mL respectively using different concentration of 1 g/L, 4 g/L, 0.5 g/L, 3.5% v/v and 1% v/v respectively through one factor at a time approach. Response surface methodology was employed to examine the interaction of critical medium components and their impact on biocatalyst activity. The range and level of selected independent variables were explored using the Box-Behnken experimental design. The maximum production was observed at the 6th run, reaching 13.0 U/mL by using combination of various critical medium components i.e., sucrose 3 g/L, molasses 5 g/L, yeast extract 1.5 g/L, sunflower oil 2.25% v/v, and Tween-80 0.5% v/v. Regarding the basal quantity of substrate, 10 g of mustard meal was also utilized.