A metagenomics-based approach to decipher the resistome and mobilome of two seahorse species, Hippocampus barbouri and Hippocampus comes

IF 4.9 2区 医学 Q1 INFECTIOUS DISEASES International Journal of Antimicrobial Agents Pub Date : 2024-08-03 DOI:10.1016/j.ijantimicag.2024.107296
Chinee S. Padasas-Adalla , Rose Chinly Mae H. Ortega-Kindica , Carlos O. Lomelí-Ortega , Sharon Rose M. Tabugo , José Luis Balcázar
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Abstract

Objective

This study aimed to explore the abundance and diversity of antibiotic resistance genes (ARGs) in seahorses (Hippocampus barbouri and Hippocampus comes) and their surrounding environment.

Methods

A combination of shotgun metagenomics and bioinformatics was used to investigate the resistome of both seahorse species.

Results

The analyses demonstrated a higher abundance of ARGs in seahorse-associated microbiomes, particularly in skin and gut samples, compared to those from water and sediment. Interestingly, genes conferring multidrug resistance (e.g., acrB, acrF, cpxA, msbA, and oqxB) were highly prevalent in all samples, especially in skin and gut samples. High levels of genes conferring resistance to fluoroquinolones (e.g., mfd and emrB), β-lactam (e.g., blaCMY-71, blaOXA-55, and penA), aminocoumarin (e.g., mdtB and mdtC), and peptide antibiotics (arnA, pmrE, and rosA) were also observed in skin and gut samples. An enrichment of mobile genetic elements (MGEs) was also observed in the analysed samples, highlighting their potential role in facilitating the acquisition and spread of ARGs. In fact, the abundance of mobilisation (MOB) relaxases (e.g., MOBF, MOBP, MOBT, and MOBV) in gut and skin samples suggests a high potential for conjugation events.

Conclusions

The occurrence of ARGs and MGEs in seahorses and the surrounding environment raises concerns about their transmission to humans, either through direct contact or the consumption of contaminated seafood. To the best of our knowledge, this study represents the first comprehensive analysis of ARGs in seahorse-associated microbiomes, and its results emphasise the need for monitoring and controlling the spread of ARGs in environmental settings.

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基于元基因组学的方法解密两种海马--Hippocampus barbouri 和 Hippocampus comes 的抗性基因组和动员基因组。
本研究旨在探索海马(Hippocampus barbouri和Hippocampus comes)及其周围环境中抗生素耐药基因(ARGs)的丰度和多样性。霰弹枪元基因组学和生物信息学相结合的研究表明,与水和沉积物相比,海马相关微生物组(尤其是皮肤和肠道样本)中的 ARGs 丰度更高。有趣的是,赋予多重耐药性的基因(如acrB、acrF、cpxA、msbA 和 oqxB)在所有样本中都非常普遍,尤其是在皮肤和肠道样本中。在皮肤和肠道样本中还观察到对氟喹诺酮类(如 mfd 和 emrB)、β-内酰胺类(如 blaCMY-71、blaOXA-55 和 penA)、氨基香豆素类(如 mdtB 和 mdtC)和肽类抗生素(arnA、pmrE 和 rosA)具有高水平抗性的基因。在分析的样本中还观察到富集的移动遗传因子(MGEs),这突显了它们在促进 ARGs 获取和传播方面的潜在作用。事实上,肠道和皮肤样本中存在大量的动员(MOB)弛豫酶(如 MOBF、MOBP、MOBT 和 MOBV),这表明发生共轭事件的可能性很大。海马和周围环境中出现的 ARGs 和 MGEs 引起了人们对其通过直接接触或食用受污染海产品传播给人类的担忧。据我们所知,本研究是首次对海马相关微生物组中的 ARGs 进行全面分析,其结果强调了监测和控制 ARGs 在环境中传播的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
21.60
自引率
0.90%
发文量
176
审稿时长
36 days
期刊介绍: The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.
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