Effects of smokeless nicotine on blood physiology, biochemical, and histological alterations using Labeo rohita as a model organism.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-06-19 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k796
Shabbir Ahmad, Hasnain Akmal, Sajid Ali, Kamran Jafar, Muhammad Shoaib, Muqadas Shahzadi, Iqra Akram, Taqi Shahid Jaffari, Irfan Ahmad, Arva Mehmood, Khurram Shahzad
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Abstract

Objective: The present research was conducted to evaluate the negative effects of nicotine powder on the blood physiology, and biochemical and histological alterations of Labeo rohita.

Materials and methods: Fish were divided into four groups (1-4). Fish groups 2, 3, and 4 were exposed to different concentrations of nicotine, such as 0.75, 1.25, and 1.75 mg/l, while group 1 acted as a control. To find out the long-term impact of nicotine on body physiology, we conducted a 42-day experiment. After the completion of the experiment, hematology, biochemical assays, and histology were done.

Results: Results revealed a considerable increase in HGB, red blood cells, WBCs, hematocrit, mean corpuscular volume, red cell distribution width -SD, procalcitonin, neutrophils, lymphocytes, monocytes, triglycerides, total cholesterol, low-density lipoprotein, very low-density lipoprotein, alanine transaminase, aspartate aminotransferase, globulin, thyroid stimulating hormone, BUN, creatinine, and blood glucose levels, whereas mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, RDW, platelet, high-density lipoprotein, albumin, total proteins, and T3 levels were significantly (p ≤ 0.05) decreased in exposed fish as compared to control group fish. Histological alterations showed that exposure to smokeless nicotine causes deleterious and degenerative effects in the liver, kidney, and gills of exposed fish.

Conclusion: Nicotine administration in fish results in adverse effects on different biochemical and hematological parameters and causes histological alterations in some vital organs of exposed fish.

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以鲮鱼为模型生物,研究无烟尼古丁对血液生理、生化和组织学变化的影响。
研究目的本研究旨在评估尼古丁粉对鲈鱼血液生理、生化和组织学变化的负面影响:将鱼分为四组(1-4)。材料:将鱼分为四组(1-4),第 2、3 和 4 组分别接触不同浓度的尼古丁,如 0.75、1.25 和 1.75 毫克/升,第 1 组为对照组。为了了解尼古丁对身体生理的长期影响,我们进行了为期 42 天的实验。实验结束后,我们进行了血液学、生化检测和组织学检查:结果:结果显示,HGB、红细胞、白细胞、血细胞比容、平均血球容积、红细胞分布宽度-SD、降钙素原、中性粒细胞、淋巴细胞、单核细胞、甘油三酯、总胆固醇、低密度脂蛋白、极低密度脂蛋白、丙氨酸转氨酶、天门冬氨酸转氨酶、胰高血糖素、胰高血糖素、胰高血糖素、胰高血糖素、胰高血糖素、胰高血糖素、胰高血糖素、胰高血糖素而平均体细胞血红蛋白浓度、平均体细胞血红蛋白、RDW、血小板、高密度脂蛋白、白蛋白、总蛋白和 T3 水平则显著下降(p ≤ 0.05)。组织学变化表明,暴露于无烟尼古丁会对暴露鱼的肝脏、肾脏和鳃造成有害和变性影响:结论:在鱼体内施用尼古丁会对不同的生化和血液学参数产生不利影响,并导致暴露鱼的一些重要器官发生组织学改变。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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