Growth inhibitory effect of Leptospermum scoparium (manuka) chloroform extract on breast and liver cancer cell lines.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-06-04 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k769

Mohammed Al-Zharani

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Abstract

Objective: Research has demonstrated that Leptospermum scoparium possesses various therapeutic benefits. This study set out to determine whether or not L. scoparium extracts had any effect on the ability of HepG2 and MCF-7 breast cancer cells to survive.

Materials and methods: The antiproliferative activity of L. scoparium extracts was explored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. The most active fraction was selected to investigate its effects on apoptosis induction using flow cytometry and quantitative real-time polymerase chain reaction. The constituents of this fraction were characterized using GC-MS analysis.

Results: Research demonstrated that the chloroform fraction of L. scoparium (LSCF) significantly impacted the HepG2 and MCF-7 cancer cell lines. Treatment with LSCF led to a notable rise in both early and late apoptotic cells. Furthermore, there was an upregulation in the mRNA levels of P53, Bax, and caspases, while the expression of Bcl-2 mRNA saw a decrease. The analysis of LSCF revealed the primary components to be cis-calamenene, beta-eudesmol, cyclododecane, and alpha-muurolene.

Conclusion: The study showed the promising antiproliferative activity of L. scoparium, suggesting its potential application for cancer treatment.

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Leptospermum scoparium（麦卢卡）氯仿提取物对乳腺癌和肝癌细胞株的生长抑制作用。

目的：研究表明，Leptospermum scoparium 具有多种治疗功效。本研究旨在确定 L. scoparium 提取物是否对 HepG2 和 MCF-7 乳腺癌细胞的存活能力有影响：使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑和乳酸脱氢酶检测法探讨莨菪提取物的抗增殖活性。利用流式细胞仪和定量实时聚合酶链反应，选择了活性最强的馏分来研究其对诱导细胞凋亡的作用。利用气相色谱-质谱分析法对该馏分的成分进行了表征：研究表明，莨菪的氯仿馏分（LSCF）对 HepG2 和 MCF-7 癌细胞株有显著影响。用 LSCF 处理后，早期和晚期凋亡细胞都明显增加。此外，P53、Bax 和 caspases 的 mRNA 水平出现上调，而 Bcl-2 mRNA 的表达则出现下降。对 LSCF 的分析表明，其主要成分是顺式菖蒲烯、β-桉叶油醇、环十二烷和α-室栾烯：这项研究表明，鳞叶甘菊具有良好的抗增殖活性，有望应用于癌症治疗。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.