Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line.

IF 2.1 Q3 PHARMACOLOGY & PHARMACY Advances in Pharmacological and Pharmaceutical Sciences Pub Date : 2024-07-25 eCollection Date: 2024-01-01 DOI:10.1155/2024/8885068
Ebtisam Yousef A Alnakeeb, Ahmad Faizal Abdull Razis, Kim Wei Chan, Chau Ling Tham, Yee Han Chan, Anwar Salm Kalifa Kafo, Nuzul Noorahya Jambari, Patrick Rollin, Florence Djedaini-Pilard
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Abstract

Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p < 0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.

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大鼠嗜碱性白血病(RBL-2H3)细胞系中的α-环糊精莫林素复合物的抗过敏作用
过敏性疾病(ADs)是关系到公众健康的一个主要问题。油杉是一种热带植物,因含有异硫氰酸酯而被用于传统医药中。本研究调查了 4-(α-L-鼠李糖基氧基)-苄基异硫氰酸酯或从油辣木籽中分离出的α-环糊精-oringin(α-CD/MG)复合物对大鼠嗜碱性白血病(RBL-2H3)细胞系在过敏反应早期和晚期的抗过敏特性。α-CD/MG复合物最初是用核磁共振(NMR)来阐明的,然后用3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺酸苯基)-2H-四唑内盐增殖试验来评估富马酸酮替芬(KF)和α-CD/MG的细胞毒性和细胞活力。β-己糖胺酶(β-hexosaminidase)和组胺的释放被用来确定早期的抑制水平,而前列腺素(PGD2)、肿瘤坏死因子-α(TNF-α)和白细胞介素(IL-4)的释放抑制则被视为晚期的抑制水平。在肥大细胞脱颗粒过程中,较高浓度的α-CD/MG(5 μM,p < 0.001)能显著抑制β-己糖胺酶、组胺、TNF-α、PGD2 和 IL-4 在早期和晚期的表达。因此,α-CD/MG 有可能被开发成一种抗过敏药物,因为它具有抑制早期和晚期过敏反应的能力。
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来源期刊
CiteScore
4.30
自引率
3.60%
发文量
0
审稿时长
17 weeks
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