Ebtisam Yousef A Alnakeeb, Ahmad Faizal Abdull Razis, Kim Wei Chan, Chau Ling Tham, Yee Han Chan, Anwar Salm Kalifa Kafo, Nuzul Noorahya Jambari, Patrick Rollin, Florence Djedaini-Pilard
{"title":"Antiallergic Effect of the Alpha-Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL-2H3) Cell Line.","authors":"Ebtisam Yousef A Alnakeeb, Ahmad Faizal Abdull Razis, Kim Wei Chan, Chau Ling Tham, Yee Han Chan, Anwar Salm Kalifa Kafo, Nuzul Noorahya Jambari, Patrick Rollin, Florence Djedaini-Pilard","doi":"10.1155/2024/8885068","DOIUrl":null,"url":null,"abstract":"<p><p>Allergic diseases (ADs) are a major concern when it comes to public well-being. <i>Moringa oleifera</i> Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(<i>α</i>-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from <i>Moringa oleifera</i> seeds in the form of alpha-cyclodextrin-moringin (<i>α</i>-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The <i>α</i>-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and <i>α</i>-CD/MG. The release of beta-hexosaminidase (<i>β</i>-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-<i>α</i>), and interleukin (IL-4) was considered in the late stage. Higher concentrations of <i>α</i>-CD/MG (5 <i>μ</i>M, <i>p</i> < 0.001) in mast cell degranulation significantly inhibited the expression of <i>β</i>-hexosaminidase, histamine, TNF-<i>α</i>, PGD2, and IL-4 in both the early and late stages. Thus, <i>α</i>-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2024 ","pages":"8885068"},"PeriodicalIF":2.1000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300081/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Pharmacological and Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/8885068","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p < 0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.